Sequences from confirmation amplicon


Confirmation sequences for line 639A10 and hit At3g09300 are shown.
Line ID 639A10
Locus At3g09300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2858968
Primers used for PCR CH91 - 8474, show primer details
   
Sequence Name 32-K107246-0022-639-A10C-CH91
GenBank Accession LN500609 [GenBank]
Sequencing primer gene specific primer, CH91: 5'-ATTTTGATGCACTTCTAAAATGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K107246-0022-639-A10C-CH91
TTGACGATTCGTTGTGTTTTGATTTGCTTCTCAGTTTGGTTCCCTATGAGGGACTTGAAG
TTATCAATCCTGAAGGAAGTACAGATGATGCTGAGGAGGAAGCAAGCAGAGGAAGATGGA
AGCAAGAGGTATATATACTTCTTCCTTTACTATTGTTGTTGTTGACATAACTGTTGATCT
AATCATCTGAGAAATATACACAATTCTTAGGATCGAGATGGCTATTGGAAGATGATGCAG
AAGTACATAGGATCTGATGTTACATCAATGGTGACCCTTCCTGTGATTATTTTTGAACCA
ATGACAATGCTTCAGAAAATGGCGGAGGTTTGTGCTCATGATCACATCTGATGATTTGTT
CCTGTCTGATATAGTTTTGTAATCCGATGATGCCAATGATATGTGCTCTGTTTCTGGTAG
TTGATGGAATACTCGCATCTGCTAGACATGGCAGACAAAACCGAGGACCCTTATTTGCGC
ATGGTGTATGCATGTAAGAACATTTTCCCTCCTTTGATTTCTTCTATGATCAGTACGTTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACG
GTTATTA
   
Sequence Name 16-K107245-0022-639-A10C-8409
GenBank Accession LN493456 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K107245-0022-639-A10C-8409
CGGAATGAGCCTTTACAACGTACTGATCATAGAAGAAATCAAAGGAGGGAAAATGTTCTT
ACATGCATACACCATGCGCAAATAAGGGTCCTCGGTTTTGTCTGCCATGTCTAGCAGATG
CGAGTATTCCATCAACTACCAGAAACAGAGCACATATCATTGGCATCATCGGATTACAAA
ACTATATCAGACAGGAACAAATCATCAGATGTGATCATGAGCACAAACCTCCGCCATTTT
CTGAAGCATTGTCATTGGTTCAAAAATAATCACAGGAAGGGTCACCATTGATGTAACATC
AGATCCTATGTACTTCTGCATCATCTTCCAATAGCCATCTCGATCCTAAGAATTGTGTAT
ATTTCTCAGATGATTAGATCAACAGTTATGTCAACAACAACAATAGTAAAGGAAGAAGTA
TATATACCTCTTGCTTCCATCTTCCTCTGCTTGCTTCCTCCTCAGCATCATCTGTACTTC
CTTCAGGATTGATAACTTCAAGTCCCTCATAGGGAACCAAACTGAGAAGCAAATCAAAAC
ACAACGAATCGTTCAAAAGAGCTTAACTTTAAGCAAAGCCCATTTTAGAAGTGAATCAAA
A

Last Updated on 06.12.2018 16:11