Sequences from confirmation amplicon


Confirmation sequences for line 639D02 and hit At3g13020 are shown.
Line ID 639D02
Locus At3g13020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4168329
Primers used for PCR 29lx - 8474, show primer details
   
Sequence Name 73-K114129-0022-639-D02C-29lx
GenBank Accession LN512947 [GenBank]
Sequencing primer gene specific primer, 29lx: 5'-TTTCATCGTCTTAAACACCACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K114129-0022-639-D02C-29lx
AGTTTCACTTACTCTTAGAGAGACGTTTCGAACGATGCTTATGGAGGATAAATCTGGTTA
TACTACTCCTAAGACTAAGAGAGTTGGGAAATTCCAAATGGCTGATTCGCGTAAGCGTCG
GAAGACCGAAGATTCATCAAGCAAAAGTGTTTCTCCGGAGCAGGGGAATGTAGCTGTGGA
AGTTGACAATCAGGATTTGTTGTCAAGCAAAGCCCAAAAGTGCATTGGTCGGTTCTTTTA
TGAACATTGCGTCGACCTTTCAGCTGTGGATTCCCCATGTTTCAAAGAAATGATGATGGC
ACTAGGTGTTGGTCAGAAGATCCCTGATTCTCATGATTTAAACGGCCGGTTGCTTCAAGA
AGCAATGAAGGAAGTGCAGGATTATGTGAAGAATATTAAAGATTCCTGGAAGATCACCGG
TTGCAGCATCTACATGAAAGAAAGAGTAAATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACGATTATT
   
Sequence Name 31-K114128-0022-639-D02C-8409
GenBank Accession LN500254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114128-0022-639-D02C-8409
TCTTTCTTTCTGTAGATGCTGCAACCGGTGATCTTCCAGGAATCTTTAATATTCTTCACA
TAATCCTGCACTTCCTTCATTGCTTCTTGAAGCAACCGGCCGTTTAAATCATGAGAATCA
GGGATCTTCTGACCAACACCTAGTGCCATCATCATTTCTTTGAAACATGGGGAATCCACA
GCTGAAAGGTCGACGCAATGTTCATAAAAGAACCGACCAATGCACTTTTGGGCTTTGCTT
GACAACAAATCCTGATTGTCAACTTCCACAGCTACATTCCCCTGCTCCGGAGAAACACTT
TTGCTTGATGAATCTTCGGTCTTCCGACGCTTACGCGAATCAGCCATTTGGAATTTCCCA
ACTCTCTTAGTCTTAGGAGTAGTATAACCAGATTTATCCTCCATAAGCATCGTTCGAAAC
GTCTCTCTAAGAGTAAGTGAAACTTGGTCACAGTGAGTAACATCAGTACCAACAGCACCC
AAGTGGTGTTTAAACCGATGAA

Last Updated on 06.12.2018 16:11