Sequences from confirmation amplicon


Confirmation sequences for line 639E08 and hit At2g38530 are shown.
Line ID 639E08
Locus At2g38530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16128696
Primers used for PCR SD32 - 8474, show primer details
   
Sequence Name 51-K103548-0022-639-E08C-SD32
GenBank Accession LN507475 [GenBank]
Sequencing primer gene specific primer, SD32: 5'-ACGTTGTTTGCGAACATAGTACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103548-0022-639-E08C-SD32
GATAAAAAGACGGAAATTGAAAAGAGTACAGCCATTCGAACATCTTTATTTAATATTATC
TCATCGATTATATAATATTCGAAACATCCGCTAGTAGCTTCATTTGACCGTCGCTCATCA
CCTCACGCTGCATTCACATACAAATTAACATAACAACGTTAATATAAGTTTCCAATATTT
AACAAAATAAACTAAAAAATTTATAACATATACTTATAAGAGTGAAAAGATCACATACGT
GTTGCAGTTGGTGCTGGCGCTGATTTTGTAAGGAATATTGACTTTGCATGCGCTAGGAAG
TCCAGCTGCACGGGCAGTGTTGAGACCGGGACCAACGGCTTTAGCGGCAGATTGAAGGCA
ACGGCAAGCTTGCTGACGGTCTGGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACGATTTATT
   
Sequence Name 35-K103549-0022-639-E08C-8409
GenBank Accession LN501801 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K103549-0022-639-E08C-8409
ACGTCGCAGCTTGCCGTTGCCTTCATCTGCCGCTAAGCCGTTGGTCCCGGTCTCAACACT
GCCCGTGCAGCTGGACTTCCTAGCGCATGCAAAGTCAATATTCCTTACAAAATCAGCGCC
AGCACCAACTGCAACACGTATGTGATCTTTTCACTCTTATAAGTATATGTTATAAATTTT
TTAGTTTATTTTGTTAAATATTGGAAACTTATATTAACGTTGTTATGTTAATTTGTATGT
GAATGCAGCGTGAGGTGATGAGCGACGGTCAAATGAAGCTACTAGCGGATGTTTCGAATA
TTATATAATCGATGAGATAATATTAAATAAAGATGTTCGAATGGCTGTACTCTTTTCAAT
TTCCTGTCTTTTTTTATCGTGGCGTTTCTATTATGTGATCGTCTGTACTATGTTCGCAAA
CAACGT

Last Updated on 06.12.2018 16:11