Sequences from confirmation amplicon


Confirmation sequences for line 639F05 and hit At5g27320 are shown.
Line ID 639F05
Locus At5g27320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9630173
Primers used for PCR JV08 - 8474, show primer details
   
Sequence Name 70-K032929-022-639-F05C-8409
GenBank Accession LN512313 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K032929-022-639-F05C-8409
ATTTACAATTGAATATAGGGTATCGATTCTCTGGTGCACGACGATAATTCACAGAGACAA
CAACAGCACCACACAAACCAACAAGCCTACGACAAAGAGTATCATAAATAGCACTGTTTG
CAGAAGAATGTGCAAAGCTTCCACCATGAAAGAAGACAATAACAGGCACTATTTCACCAT
CAACAGGATTCTGTAGATCAGTAATACTTGGTGAAGTGCCAGCATCAGCTGGTCTGTAAA
CTCTGCTAAGCAAATTAGTTTGGCGATCGATGATCACATCAAAAGAGAAGACCCCATTAA
CAGGGTTGGCATTTGCAGGGACTTTCCGGTCTAGAAACTCTGCGAGATGACGGTTAAAAG
TTCCGTCAGGGCGACGCAGGAGATTGTAAGCTAGCTTAAAGTTGGATATTAGAACCCATG
TATTGAGAGGAACCAACTGTCT
   
Sequence Name 70-K032929-022-639-F05C-JV08
GenBank Accession LN512314 [GenBank]
Sequencing primer gene specific primer, JV08: 5'-CAGACAGTGGTTCCTCTCAATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K032929-022-639-F05C-JV08
ACTTTAGCTAGCTACATCTCCTGCGTCGCCCTGACGGAACTTTTAACCGTCATCTCGCAG
AGTTTCTAGACCGGAAAGTCCCTGCAAATGCCAACCCTGTTAATGGGGTCTTCTCTTTTG
ATGTGATCATCGATCGCCAAACTAATTTGCTTAGCAGAGTTTACAGACCAGCTGATGCTG
GCACTTCACCAAGTATTACTGATCTACAGAATCCTGTTGATGGTGAAATAGTGCCTGTTA
TTGTCTTCTTTCATGGTGGAAGCTTTGCACATTCTTCTGCAAACAGTGCTATTTATGATA
CTCTTTGTCGTAGGCTTGTTGGTTTGTGTGGTGCTGTTGTTGTCTCTGTGAATTATCGTC
GTGCACCAGAGAATCGATACCCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11