Sequences from confirmation amplicon


Confirmation sequences for line 640B09 and hit At1g50600 are shown.
Line ID 640B09
Locus At1g50600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18737557
Primers used for PCR KQ61 - 8474, show primer details
   
Sequence Name 50-K101565-0022-640-B09C-8409
GenBank Accession LN507112 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101565-0022-640-B09C-8409
GCCATTTACATTGAATATATCCTGATAAACGGTCATGTGAAACCGAGACCTCCATTTCCC
TAGTGGCTCGTGCCTCTCTTCTCTTTCAACACCTTCACAAGCTATAAGATTCACAACCTC
TCTAGCCAAACAATGCTGCTCAACATTGATCCTTTCCTTGTGATCTCTAGCGAGTTTCAC
ATCTATTGATTCGAAAACTGCCAAGTAATGGTTCATTGTCTCGACAAACCGGGGAAGAAA
CGGCGCAGTGTTTGTATTCGCTTCTTGCTCAACCAGAGTCACAACGTTTGGTGACAAGTG
TTTGACCAATCTCAACAATCTATCTCTGTGATTCTCCACAGTTACACTCTCATCAGGCAT
GTGGTGAAGAACAAGCGGGAAGTTAACCGCGAGCGCTTCTCCATTTCTAACTCCTAGCTT
CTCGATTTCGACTTCCGTGCAGCATAAAGCAGCTCCATGGAACTCAAACGGAACACCGCA
CATTTCAGCTAGCTTCCCAAGTCTTTGTCCAACTAACTC
   
Sequence Name 50-K101578-0022-640-B09C-KQ61
GenBank Accession LN507113 [GenBank]
Sequencing primer gene specific primer, KQ61: 5'-AGGAGGACTTGAGTTAGTTGGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101578-0022-640-B09C-KQ61
GCGGTGTTCCGTTTGAGTTCCATGGAGCTGCTTTATGCTGCACGGAAGTCGAAATCGAGA
AGCTAGGAGTTAGAAATGGAGAAGCGCTCGCGGTTAACTTCCCGCTTGTTCTTCACCACA
TGCCTGATGAGAGTGTAACTGTGGAGAATCACAGAGATAGATTGTTGAGATTGGTCAAAC
ACTTGTCACCAAACGTTGTGACTCTGGTTGAGCAAGAAGCGAATACAAACACTGCGCCGT
TTCTTCCCCGGTTTGTCGAGACAATGAACCATTACTTGGCAGTTTTCGAATCAATAGATG
TGAAACTCGCTAGAGATCACAAGGAAAGGATCAATGTTGAGCAGCATTGTTTGGCTAGAG
AGGTTGTGAATCTTATAGCTTGTGAAGGTGTTGAAAGAGAAGAGAGGCACGAGCCACTAG
GGAAATGGAGGTCTCGGTTTCACATGACCGTTTATCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATAACCGCA

Last Updated on 06.12.2018 16:11