Sequences from confirmation amplicon


Confirmation sequences for line 640H10 and hit At1g14940 are shown.
Line ID 640H10
Locus At1g14940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5155212
Primers used for PCR NS27 - 8474, show primer details
   
Sequence Name 80-K102370-0022-640-H10C-8409
GenBank Accession LN513932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K102370-0022-640-H10C-8409
GTAGCTGGCCTCAACCTTCTCAAACTCTCCTCCAGAGTTTCAAAGTAGGGCTACCGACAC
CGGGTGTCCACTCTCTTTAATCATGTTTGGTAATACGAGGATTGACGATCGGCTAATTTG
GTCTAGCATTGTGTGTGTGTGTGTTTGTATGAAGATGGAAAGCCGGAAGTCTTCAAGGAG
AAGAGAGAGATAGACGACGAAAAGATGGCGTTGACGTTGAAAGGGCTAGAGGGTCAAGCG
ATGGAGAAGTACAAGAAGTATGAGGTTATCTACCAGTTCATTCCCAAGTCGAAGGAAGGC
TGCGTCTGCAAAATCACTCTCATATGGGAAAAGCGCAACGAAAACTCCCCCGAACCCATC
AACTACATGAAGTTTGTCAAGAGCTTAGTTGCTGACATGGACGACCACGTCCTCAATGGC
CAGAACAAAGCTTGAATCCCCTCTATGGTCATCGTCGAATGGTCTTCATCTTTATGTGTG
TGTATCTTTTGTGTATCTTTACGTACGTACGTGACTTATCATCCGCCTCTTTCCCCCTTT
ATACATGTTATGCTAATAATAATGGCGGTCTTGGA
   
Sequence Name 80-K102371-0022-640-H10C-NS27
GenBank Accession LN513933 [GenBank]
Sequencing primer gene specific primer, NS27: 5'-TTCCAAGACCGCCATTATTATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K102371-0022-640-H10C-NS27
GGGGGAAGAGCGGATGATAGTCACGTACGTACGTAAAGATACACAAAAGATACACACACA
TAAAGATGAAGACCATTCGACGATGACCATAGAGGGGATTCAAGCTTTGTTCTGGCCATT
GAGGACGTGGTCGTCCATGTCAGCAACTAAGCTCTTGACAAACTTCATGTAGTTGATGGG
TTCGGGGGAGTTTTCGTTGCGCTTTTCCCATATGAGAGTGATTTTGCAGACGCAGCCTTC
CTTCGACTTGGGAATGAACTGGTAGATAACCTCATACTTCTTGTACTTCTCCATCGCTTG
ACCCTCTAGCCCTTTCAACGTCAACGCCATCTTTTCGTCGTCTATCTCTCTCTTCTCCTT
GAAGACTTCCGGCTTTCCATCTTCATACAAACACACACACACACAATGCTAGACCAAATT
AGCCGATCGTCAATCCTCGTATTACCAAACATGATTAAAGAGAGTGGACACCCGGTGTCG
GTAGCCCTACTTTGAAACTCTTGGAGGAGAGTTTGAGAAGGTTCGAGGCCTAGCTACCAA
CATCTGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATA

Last Updated on 06.12.2018 16:11