Sequences from confirmation amplicon


Confirmation sequences for line 641D05 and hit At4g10370 are shown.
Line ID 641D05
Locus At4g10370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6425528
Primers used for PCR 21ww - 8474, show primer details
   
Sequence Name 47-K118874-0022-641-D05C-21ww
GenBank Accession LN506348 [GenBank]
Sequencing primer gene specific primer, 21ww: 5'-CTCAAATTGCAGGTAAGACAATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118874-0022-641-D05C-21ww
CAATGCAAACTCAGGCGCTGGATGATAGTAAAGTCGAAGAGAATGAAAAAGGTGGGTTGG
GTGTTTGATTTCAAGAGGAGACTCTACACATTCTTTGTGGAATTTTTTTTCACATTGAAG
ACATGCATAATAGCTAGCGCTGAGCATTGAATCTCTGCATCCACCGCACTGGAATTCGCG
AGAATCACTTTCTTTATTGTTGCACCAAAAGAGAGGGACTACAGGGAGTTTACAGATACC
ATCTTCGTCGCAATCAAGCAAAGATTCACCTTGTTGATCACCGCTTCTTTTGCTGGGGAA
GTGAGGGGAAGAAATGTATGGATGAAACTTGAAATCATAATCAAAAATATCACCATCCTC
TCTCTGTATTTTGAGCTTGTGAAATTTGATACGTGCATCAGGACATAGAAAAAGAGGTTG
ATCAAGAGGTTGGTCGTCGCCAGTGGAATCCATGTAAATGGCTTCATGGAAGGCGGTATC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
AATAGATGTCC
   
Sequence Name 07-K118873-0022-641-D05C-8409
GenBank Accession LN489491 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118873-0022-641-D05C-8409
CATTTCATTGAATAATCCTGATACCGCCTTCCATGAAGCCATTTCCCTATATTCCACTGG
CGACGACCAACCTCTTGATCAACCTCCTTTTCTATGTCCTGATGCACGTATCAAATTTCA
CAAGCTCAAAATACAGAGAGAGGATGGTGATATTTTTGATTATGATTTCAAGTTTCATCC
ATACATTTCTTCCCCTCACTTCCCCAGCAAAAGAAGCGGTGATCAACAAGGTGAATCTTT
GCTTGATTGCGACGAAGATGGTATCTGTAAACTCCCTGTAGTCCCTCTCTTTTGGTGCAA
CAATAAAGAAAGTGATTCTCGCGAATTCCAGTGCGGTGGATGCAGAGATTCAATGCTCAG
CGCTAGCTATTATGCATGTCTTCAATGTGAAAAAAAATTCCACAAAGAATGTGTAGAGTC
TCCTCTTGAAATCAAACACCCAACCCACCTTTTTCATTCTCTTCGACTTTACTATCATCC
AGCGCCTGAGTTTTGCATTTGTTGTAAAACAGAAGTCTTCATGATTTTCTATCATTGTCT
TACCGCAAATTTGA

Last Updated on 06.12.2018 16:11