Sequences from confirmation amplicon


Confirmation sequences for line 643A05 and hit At1g20880 are shown.
Line ID 643A05
Locus At1g20880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7264887
Primers used for PCR 11im - 8474, show primer details
   
Sequence Name 52-K113845-0022-643-A05C-11im
GenBank Accession LN507881 [GenBank]
Sequencing primer gene specific primer, 11im: 5'-TCCTTTTGTTTTCTCTCTCTCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113845-0022-643-A05C-11im
AAAACTTCCATAAACAAATCCTTCCTCGTCCCCTCGTATTTTTCTATCTAAATCCCTAAA
ACCACTCAAAATCATCCCTCTTTCTCTCCTCCCCATTTTCCCCAACGCTTGATTCGAATT
CGTCTCTCTCGTTTTCTGTTGTTCTACCAATTGAGCAACCCTAGAATCATTCTTGTGTAC
CTACCGGAACCGAGTCTTCTCTTCTTCCGTGGCACATCTTTCAAAGTTTTAAACCCCTTT
TCCCCTAACTTCTTTTCATTTTTCTGTTTTTTTCCCGGGAAATTTTCTCTCTCTCTCTCT
ATTTTTTGTTTCTCTCGTCATGATCATTACTTGTCAAGTATGATCCTTTATTAGGTTTCT
TTGGGGCCGATCTCACTTTTTCTGTTTCTCACCCTCTTCTCTTCGAAGGCCCTTGTAAAC
CCTAGAATCTCCTCTTTTCAAATTTATATGTTCTGTTTCTTCATCTTCAGGTTTAATCAT
TCAATCTATCTCTAGGGTTTACCGGGTCAAGTCTTAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 25-K113844-0022-643-A05C-8409
GenBank Accession LN497533 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K113844-0022-643-A05C-8409
TAAGAACTTGACCCGGTAAACCCTAGAGATAGATTGAATGATTAAACCTGAAGATGAAGA
AACAGAACATATAAATTTGAAAAGAGGAGATTCTAGGGTTTACAAGGGCCTTCGAAGAGA
AGAGGGTGAGAAACAGAAAAAGTGAGATCGGCCCCAAAGAAACCTAATAAAGGATCATAC
TTGACAAGTAATGATCATGACGAGAGAAACAAAAAATAGAGAGAGAGAGAGAAAATTTCC
CGGGAAAAAAACAGAAAAATGAAAAGAAGTTAGGGGAAAAGGGGTTTAAAACTTTGAAAG
ATGTGCCACGGAAGAAGAGAAGACTCGGTTCCGGTAGGTACACAAGAATGATTCTAGGGT
TGCTCAATTGGTAGAACAACAGAAAACGAGAGAGACGAATTCGAATCAAGCGTTGGGGAA
AATGGGGAGGAGAGAAAGAGGGATGATTTTGAGTGGTTTTTAGGGATTTAGATAGAAAAA
TACAAGGGGACAAGGAAGGATTTGTTTATGGAAGTTTTAACATTCATGATGATGGTGAAG
AAGTTGGTGATGACNAAGACGATGATGCGTGATTATGATTCATAAATTCTTCGAGAGAGA
GAG

Last Updated on 06.12.2018 16:11