Sequences from confirmation amplicon


Confirmation sequences for line 643F07 and hit At2g31880 are shown.
Line ID 643F07
Locus At2g31880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13555924
Primers used for PCR HV53 - 8474, show primer details
   
Sequence Name 74-K101386-0022-643-F07C-8409
GenBank Accession LN513038 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K101386-0022-643-F07C-8409
CGGACTGAGCCTTTACATTGAATATATCCATGAAGATTTAGCTTTCTTGGAGAATGAAGA
AGCGTTAGCTTCTCTGGAGATCATAGGAAGAGGAGGTTGTGGAGAAGTTTTCAAAGCTGA
ACTACCAGGAAGCAATGGGAAGATCATAGCTGTGAAGAAAGTGATCCAACCGCCTAAAGA
CGCCGATGAACTAACAGATGAAGATTCCAAGTTTCTGAACAAGAAAATGAGGCAAATTAG
ATCCGAGATCAACACAGTCGGCCATATCCGGCACCGGAATCTTCTCCCATTGTTAGCACA
CGTGTCAAGACCCGAGTGCCACTACCTCGTTTACGAGTACATGGAANAGGGGAGTTTGCA
AGATATATTGACTGACGTACAAGCTGGAAATCAAGAACTAATGTGGCCAGCGAGGCACAA
GATTGCTTTAGGTATAGCTGCAGGGCTTGAGTACCTTCACATGGATCATAACCCACGAAT
CATTCACAGAGACTTAAAGCCAGCCCATGTTCTTCTTGATGATGACATGGAAG
   
Sequence Name 74-K101392-0022-643-F07C-HV53
GenBank Accession LN513039 [GenBank]
Sequencing primer gene specific primer, HV53: 5'-AGGCTAGGATTTTCCGATGTTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K101392-0022-643-F07C-HV53
TCTCGTCGTATGCTGAAAAACTCATCTGAAGGAAGCTTCCCAATCACCAGAATCCCAAGA
ATCACTCCAAAACTATAGATATCACATTTATCTGTGAACTTGTGGGTTTGATAAAACTCT
GGTGCTATGTATCCCACAGTACCTGCAACATGCGAGGTTGTAATATGTGTGACTGCATCT
GGCATTGCCTTTGCCAGTCCGAAATCTGAAATTCTGGCTTCCATGTCATCATCAAGAAGA
ACATTGGCTGGCTTTAAGTCTCTGTGAATGATTCGTGGGTTATGATCCATGTGAAGGTAC
TCAAGCCCTGCAGCTATACCTAAAGCAATCTTGTGCCTCGCTGGCCACATTAGTTCTTGA
TTTCCAGCTTGTACGTCAGTCAATATATCTTGCANACTCCCCTTTTCCATGTACTCGTAA
ACGAGGTAGTGGCACTCGGGTCTTGACACGTGTGCTAAACAATGGAGAAGATTCCGGTGC
CGGATATGGCCGACTGTGTTGATCTCG

Last Updated on 06.12.2018 16:11