Sequences from confirmation amplicon


Confirmation sequences for line 643G01 and hit At2g36630 are shown.
Line ID 643G01
Locus At2g36630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15354409
Primers used for PCR 34ny - 8474, show primer details
   
Sequence Name 09-K114782-0022-643-G01C-34ny
GenBank Accession LN490296 [GenBank]
Sequencing primer gene specific primer, 34ny: 5'-GGAGACACAACAAACCCTATTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114782-0022-643-G01C-34ny
TTTGGCCGGTAAATAAAAAGATTCTTCCTTTCTTCTTCTGAATATTCCATCGTCATTATT
CATTAACTTGAGTCCTTTTCAATTAGTCTTTGATTGTTTTTTAATTTGATTTATGTTATA
ATATATTTTGCTTACGTAACAACATTAATTAAAAATACACCTTTTACCCAAAAGCTTGGA
AGAATCTTTTAAAAGTGGAATTTGACTTTTGTTTGTTTTGTATGAAATTGTGTAAGTAGG
ATTTGAAGTTTAGCTGGAAACTTGTGTTGGCTACAGTGATAGCGTTTCTAGGATCAGCTT
GTGGGACTGTTGGTGGTGTTGGAGGTGGTGGGAT
   
Sequence Name 09-K114769-0022-643-G01C-8409
GenBank Accession LN490295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114769-0022-643-G01C-8409
CCCATTATCATACCTTTCACCACCAAACAACATACCAAATTAACTCAAGCATTCTCTCAT
GAAAGATCTATACAATGATTGATGAATTCATTACATTTTGATATTGCAGCTGCGGATTTT
GTATCGAACCCGAGTATAAGAGTGAGCATAGGAACAAAAATTCCACCCACCTCCNACACC
ACCAACAGTCCCACAAGCTGATCCTAGAAACGCTATCACTGTAGCCAACACAAGTTTCCA
GCTAAACTTCAAATCCTACTTACACAATTTCATACAAAACAAACAAAAGTCAAATTCCAC
TTTTAAAAGATTCTTCCAAGCTTTTGGGTAAAAGGTGTATTTTTAATTAATGTTGTTACG
TAAGCAAAATATATTATAACATAAATCAAATTAAAAAACAATCAAAGACTAATTGAAAAG
GACTCAAGTTAATGAATAATGACGATGGAATATTCAGAAGAAGAAAGGAAGAATCTTTTT
AATTTACCGGCCAAATTTTTTCGGTGGCAGAGAGAGAAGAGAGATGATGATGAA

Last Updated on 06.12.2018 16:11