Sequences from confirmation amplicon


Confirmation sequences for line 644G01 and hit At3g15530 are shown.
Line ID 644G01
Locus At3g15530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5253641
Primers used for PCR 50fr - 8474, show primer details
   
Sequence Name 31-K111683-0022-644-G01C-50fr
GenBank Accession LN500221 [GenBank]
Sequencing primer gene specific primer, 50fr: 5'-TAGTGTCGAACCTCCAGACTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K111683-0022-644-G01C-50fr
TACGACCGACCACATCACTGTCGTTTTGAACCCACGTCATCATCTCCAATCGAGGCGGAC
TTCACGGGGCCCGGCGACGAGTTCACTGGGTTTCCGGAACGAAACGATGTGACTTCCGGC
CATAAACGCCGTGACTCGCTCCGAGACTCGTATATCTTCCATCTTTAGCTCTTGAAGACG
ACGCACGTACTCCGGCACGTGTAAGAGATCCCAAACCACACACAAACCTCCTGGCTTCAC
AACTCTCACAATCTCTCCGAGCACTCTCATTCTCTCCGCCGCAGCTTCCACCGACTTCTG
ACCATGCTCTTTCCCCACCGTGTGAACGAACACCGACGAGACCACCACGTCGAAGTAATT
ATCGCCAAACGGCAACGTCTTCACATCTCCTTCACGGCATGTCACATACTCTTGCACACC
TACACAATGAGAGTACTCAAGCCAGTCACGGCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGGTTATT
   
Sequence Name 15-K111682-0022-644-G01C-8409
GenBank Accession LN493032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K111682-0022-644-G01C-8409
TTGAATATATCCTGCCGTGACTGGCTTGAGTACTCTCATTGTGTAGGTGTGCAAGAGTAT
GTGACATGCCGTGAAGGAGATGTGAAGACGTTGCCGTTTGGCGATAATTACTTCGACGTG
GTGGTCTCGTCGGTGTTCGTTCACACGGTGGGGAAAGAGCATGGTCAGAAGTCGGTGGAA
GCTGCGGCGGAGAGAATGAGAGTGCTCGGAGAGATTGTGAGAGTTGTGAAGCCAGGAGGT
TTGTGTGTGGTTTGGGATCTCTTACACGTGCCGGAGTACGTGCGTCGTCTTCAAGAGCTA
AAGATGGAAGATATACGAGTCTCGGAGCGAGTCACGGCGTTTATGGCCGGAAGTCACATC
GTTTCGTTCCGGAAACCCAGTGAACTCGTCGCCGGGCCCCGTGAAGTCCGCCTCGATTGG
AGATGATGACGTGGTGTTCAAAACGACAGTGATGTGTGTCGGTTCGTTATATAAAAAACG
GCACGTAGTTGAAGAAGTCTGGAGGTTCGACACT

Last Updated on 06.12.2018 16:11