Sequences from confirmation amplicon


Confirmation sequences for line 645A11 and hit At1g59740 are shown.
Line ID 645A11
Locus At1g59740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21969903
Primers used for PCR 25kq - 8474, show primer details
   
Sequence Name 68-K113756-0022-645-A11C-25kq
GenBank Accession LN512055 [GenBank]
Sequencing primer gene specific primer, 25kq: 5'-ACATAATCGGGATTTGTCAATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113756-0022-645-A11C-25kq
GTGTTCTTTCTTTTATACGACTACATATAAAGTGTTATGATTCCTTCGCTATAGTATAAA
ATCATAATCAACTTATTTAAAATTTTGGCTATTTAACTAGTATCCGGTAGAGACAGAAGT
CAACTCTATTGTTTATATGTGTGGTTAGTCTTAAATATATCCATATAAAATATGCTCACG
GGTCCAACTTAGATGGACTTACGTATACATACGCATGCAGACAAAATTCGAAACAGACTT
ATCTTAGAAGTCAACTATTTTTGTAGGTAAAAACCCAAGGGATCGGAATGATTTTGTAAA
GTAAATATTAATAATCATATTAAAGTATGTAGTGTAGTAAAATAGCGACGGATTATGAAT
AAATAAAAGCCATTATCAATGATTACAATATGTTCAATTACAATCTACATGGATAAGATT
TGGTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCGGTTA
TT
   
Sequence Name 30-K113755-0022-645-A11C-8409
GenBank Accession LN499803 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K113755-0022-645-A11C-8409
AATCTTATCATGTAGATTGTAATTGAACATATTGTAATCATTGATAATCGTTTTTATTTA
TTCATAATCCGTCGCTATTTTACTACACTACATACTTTAATATGATTATTAATATTTACT
TTACAAAATCATTCCGATCCCTTGGGTTTTTACCTACAAAAATAGTTGACTTCTAAGATA
AGTCTGTTTCGAATTTTGTCTGCATGCGTATGTATACGTAAGTCCATCTAAGTTGGACCC
GTGAGCATATTTTATATGGATATATTTAAGACTAACCACACATATAAACAATAGAGTTGA
CTTCTGTCTCTACCGGATACTAGTTAAATAGCCAAAATTTTAAATAAGTTGATTATGATT
TTATACTATTGCAAAGGAATCATAACACTTTATATGTAGTCGTAATAAAAGAAAGAACAC
TTTACATATTTTGCGCTAATGTTGACCCCCATATCATTGACAAATCCGNATTATGTA

Last Updated on 06.12.2018 16:11