Sequences from confirmation amplicon


Confirmation sequences for line 645E09 and hit At5g65440 are shown.
Line ID 645E09
Locus At5g65440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26154143
Primers used for PCR 09hd - 8474, show primer details
   
Sequence Name 44-K112477-0022-645-E09C-09hd
GenBank Accession LN505231 [GenBank]
Sequencing primer gene specific primer, 09hd: 5'-GGGGTTAATACATCGTTGAGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K112477-0022-645-E09C-09hd
CGTAGCAGACTCAAAAACATCTTTCATCCCGGAGAAAGACAGTTCAGCAAGTGATTTATA
ATTCTCAAATGCTGAAGCGAGAATCTGCTGAATTTGTTCGTCAATTTCCCCAAGTAACCG
ATTCTGACCAAAGGACCAAAAGTTGAAGTAAGCTACTTGAATACTGAAAATATAACAATT
TGATGATCAAAGCTAACCCACCTCTTGATGACTCAATACAGCCCTGTGGTTGCTTGTCAT
TATAATAGGGAATAGAAAATCATGTATCAGATCAAGACAGTCCTTGGTAGGCGATGCGAC
ATCCATGACATAAGAAAGATATCTGCAAGTTCTCACAAAACATCGTTTAGTTACTGCGAC
AGAAATTTAAGACACTAAAACCATTCTTAGTCACTCAATGATTACCTTAATCTAGTGTAT
GCATCTGAAACCCCGTAATAGCTACCACGGGCCATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACGGTTATTA
   
Sequence Name 21-K112476-0022-645-E09C-8409
GenBank Accession LN495727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112476-0022-645-E09C-8409
GGTTTCAGATGCATACACTAGATTAAGGTAATCATTGAGTGACTAAGAATGGTTTTAGTG
TCTTAAATTTCTGTCGCAGTAACTAAACGATGTTTTGTGAGAACTTGCAGATATCTTTCT
TATGTCATGGATGTCGCATCGCCTACCAAGGACTGTCTTGATCTGATACATGATTTTCTA
TTCCCTATTATAATGACAAGCAACCACAGGGCTGTATTGAGTCATCAAGAGGTGGGTTAG
CTTTGATCATCAAATTGTTATATTTTCAGTATTCAAGTAGCTTACTTCAACTTTTGGTCC
TTTGGTCAGAATCGGTTACTTGGGGAAATTGACGAACAAATTCAGCAGATTCTCGCTTCA
GCATTTGAGAATTATAAATCACTTGCTGAACTGTCTTTCTCCGGGATGAAAGATGTTTTT
GAGTCTGCTACGGGGACTCCAGCACCTGCTATAGAATCAGCTGTCAAGCTTTATGGTCTT
CTCAACGATGTATTAACCC

Last Updated on 06.12.2018 16:11