Sequences from confirmation amplicon


Confirmation sequences for line 645H07 and hit At2g19570 are shown.
Line ID 645H07
Locus At2g19570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8471407
Primers used for PCR BA09 - 8474, show primer details
   
Sequence Name 69-K104767-0022-645-H07C-BA09
GenBank Accession LN512200 [GenBank]
Sequencing primer gene specific primer, BA09: 5'-TGGTCCCATACTAGGATTATACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K104767-0022-645-H07C-BA09
ACCTCTGTCCTTTCCGTCCAATCCACCAGCGAAACTCCCGATGGACATAAACTATACGGC
GCGTACGATCTATTCGCCGCCGCTAAAGCCGTTTGTTTCAAATCGGCGGATGAATCGGTG
TTTCCGTTACAAATCGAATCCAGATCTGAGATTTTGAGATGGTTATCGTGAGATTCGAGA
AGAAGAGGATGATCTTTCCCGAGAAGATCGTCGGGACCGAATCTGTGTGGCAAGAAGCTT
CCGAGACGTAAGAATCCGTCTGAATCGGCGGCGGAATCGGAATCGGCGGAGTTGTTTGGA
TCGGTGATAAGGATTTTGATTTCAGGTGCGTCGCGAATTTCTTGGAGGAATTGACGGCAA
TGGCCACATGGTGCGGCGGAGACGGCGAAGAAATTGAGATGACGTTCACCGTTGAGTGTG
AGATTGGTGACGAGGAACTGTTCGGCGTGGATTGAGTGGTGGAGAGGGAGATTTGGGAAT
TCGACATTGACGCCTAAGAAGATCCGACCTGATGATCCGAGTCCGACGACTGCGACGTTG
AATTTCGAAATCGGAGTTCGAGCGTAGGATTGTGCTGGTTTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 69-K104768-0022-645-H07C-8409
GenBank Accession LN512201 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K104768-0022-645-H07C-8409
CGGATGAGCCATTTACAATTGAAACCAGCACAATCCTACGCTCGAACTCCGATTTCGAAA
TTCAACGTCGCAGTCGTCGGACTCGGATCATCAGGTCGGATCTTCTTAGGCGTCAATGTC
GAATTCCCAAATCTCCCTCTCCACCACTCAATCCACGCCGAACAGTTCCTCGTCACCAAT
CTCACACTCAACGGTGAACGTCATCTCAATTTCTTCGCCGTCTCCGCCGCACCATGTGGC
CATTGCCGTCAATTCCTCCAAGAAATTCGCGACGCACCTGAAATCAAAATCCTTATCACC
GATCCAAACAACTCCGCCGATTCCGATTCCGCCGCCGATTCAGACGGATTCTTACGTCTC
GGAAGCTTCTTGCCACACAGATTCGGTCCCGACGATCTTCTCGGGAAAGATCATCCTCTT
CTTCTCGAATCTCACGATAACCATCTCAAAATCTCAGATCTGGATTCGATTTGTAACGGA
AACACCGATTCATCCGCCGATTTGAAACAAACGGCTTTAGCGGCGGCGAATAGATCGTAC
GCGCCGTATAGTTTATGTCCATCGGGAGTTTCGCTGGTGGATTGTGACGGGAAAGTGTAC
AGAGGTTGGTATATGGAATCGGCGGCGTATAATCCTAGTATGGGACC

Last Updated on 06.12.2018 16:11