Sequences from confirmation amplicon


Confirmation sequences for line 648C11 and hit At1g43640 are shown.
Line ID 648C11
Locus At1g43640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16439625
Primers used for PCR JV15 - 8474, show primer details
   
Sequence Name 42-K033100-022-648-C11C-JV15
GenBank Accession LN504364 [GenBank]
Sequencing primer gene specific primer, JV15: 5'-AAGTCCCTTCTGGAAGTTACAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033100-022-648-C11C-JV15
TGACTTGCTTGGACCCGTGGACCTCGTAGAATGAACTGCATTATGCACTCTATCCCCTCC
TTAGCTCTAGAACCCGGAGGTACTGTCCCTAGCCAACCTGAGTTTCTACAACGTTCCCTT
GATGAATCTTTCCGCAGCATCGGTTCCTCAAAGATAGTCAACCACTCGGGAGATTTCACC
CGACCGAAAGAGGAAGAAGGAAAGGTGCGACCTTTGGTACTGAAAACTAAACCGCCAAGG
TGGCTCCAACCGTTGCGATGTTGGTGCCTTAACTTCAAAGGGAGAGTGACTGTAGCTTCT
GTCAAGAACTTCCAGTTGATGTCCGCTGCAACGGTTCAGCCCGGTAGTGGTAGTGATGGT
GGAGCATTGGCTACGAGGCCATCGTTATCACCACAGCAGCCAGAGCAATCAAACCATGAT
AAGATAATACTACACTTTGGGAAAGTGGGTAAGGATATGTTCACTATGGACTATCGTTAT
CCTCTCTCTGCCTTTCAAGCGTTTGCCATTTCCCTGAGCACCTTTGATACTAAATTGGCA
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
   
Sequence Name 90-K033100-022-648-C11C-8409
GenBank Accession LN514999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K033100-022-648-C11C-8409
TTTACATGCCACTTTAGTATCCAGGTGCTCAGGGAAATGGCAAACGCTTGAAAGGCAGAG
AGAGGATAACGATAGTCCATAGTGAACATATCCTTACCCACTTTCCCAAAGTGTAGTATT
ATCTTATCATGGTTTGATTGCTCTGGCTGCTGTGGTGATAACGATGGCCTCGTAGCCAAT
GCTCCACCATCACTACCACTACCGGGCTGAACCGTTGCAGCGGACATCAACTGGAAGTTC
TTGACAGAAGCTACAGTCACTCTCCCTTTGAAGTTAAGGCACCAACATCGCAACGGTTGG
AGCCACCTTGGCGGTTTAGTTTTCAGTACCAAAGGTCGCACCTTTCCTTCTTCCTCTTTC
GGTCGGGTGAAATCTCCCGAGTGGTTGACTATCTTTGAGGAACCGATGCTGCGGAAAGAT
TCATCAAGGGAACGTTGTAGAAACTCAGGTTGGCTAGGGACAGTACCTCCGGGTTCTAGA
GCTAAGGAGGGGATAGAGTGCATAATGCAGTTCATTCTACGAGGTCCACGGGTTCCAAGC
AAGTTCAGCTCGTATGTAACTTGAGCAATGTTGTAACT

Last Updated on 06.12.2018 16:11