Sequences from confirmation amplicon


Confirmation sequences for line 648D10 and hit At5g47820 are shown.
Line ID 648D10
Locus At5g47820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19367241
Primers used for PCR 14eb - 8474, show primer details
   
Sequence Name 27-K110124-0022-648-D10C-14eb
GenBank Accession LN498435 [GenBank]
Sequencing primer gene specific primer, 14eb: 5'-TCTTCTTCTTCTTCCAGTTTCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K110124-0022-648-D10C-14eb
ATTATCTGAACTGAGAAGAAAATCTTTTTTAGGTGAAATATATTTCAACTGATTAGCTTT
TTTTTTTTTTAAAGGGAAATGGCCCCCCCCGGAAACCAAAACCCCCCAACTTTTTAAAAA
CCGGGGACTTTTTACCGGGGTTACCTTAAGGCGGGTTGACCCAAGACCCCCGGTTTTAAG
AATAAGGGCCCCAAAAAAACCTTTCCCTTAAAAAAAAACCCCCCAAACAAATGGTTTTGG
GGGCAAATCCGAACCCCGGCCCCCCTACCAAAAAAAAATCGGAACCCCGGCCCTTTTACC
CCAAAAGGGAAATGGAAATGGGGGTTTGGAAAAAAAAAAAATTTTGGCCCCAGGCTGGTA
AAATGGTAAGAAAAAAATTTTTTTGGAAACCAAAACCAAAATTTCCTTGAAAAATGGGAT
TTTGGGAAAAAAAGGCGGGGGGCCCAAATGGGACCCACTCCTTTACCTTTGAACCCGGAA
AGGGGGACCCCGGGTCCCCCCCCCCCGGAAAGGTAAAAAAAAGGGGCCCCCCCCCTAATC
CAATGGAAAAGGGCTCCAGGCCCGGGAAACCCCCCGGGGCCCCCCA
   
Sequence Name 11-K110123-0022-648-D10C-8409
GenBank Accession LN491163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K110123-0022-648-D10C-8409
TTCGGACTGAGACATTTACATTGAATAGAGGGGCAGCACATTCTTCATACATTTCAGTAG
ATGGAGAACCAGAGCTCCCATATACATGATCAAATGTAAAAGAATGTGATCCAATTTGTA
CCTGCAGCATTATTTCACAAAAATCCAATTTTCAAAGAAATATTTGATTATGATTACAAT
AAAAATTTCTAACTAACAATTTAACAAGCATGGGCCAAAACTTATATATATTCCAAAACC
CCAATTCCAATTCACATATTGAGTAAAAAGGTCAGTGATCAGAATTTTTTTTGGTAAGGA
GGTCAGTGATCAGAATTAGCAACAAAAACAATTGATTAGTGAGCTAATTAATAAAGGAAA
AGATATTAATGATGCAATAATACTAAAAACAGAAGTACTTGGTCAAACATGCATAAAGTA
AACACTGATAAAAAGTCACTGATTCTAAAAACGTCGCGAGATCTAGATCTCAGGGAGAGA
CAATTCCGCTTAAAAAAAAAAAAAAGCAACCCGGTGAAAATTTTTTCCCCCAAAAAAAAA
TTTTTTTTCTACAGTTTCAAATTATCCAAA

Last Updated on 06.12.2018 16:11