Sequences from confirmation amplicon


Confirmation sequences for line 648F10 and hit At1g29810 are shown.
Line ID 648F10
Locus At1g29810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10437339
Primers used for PCR AG01 - 8474, show primer details
   
Sequence Name 21-K026485-022-648-F10C-8409
GenBank Accession LN495514 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K026485-022-648-F10C-8409
ACAATTCGTGTATGTTTATGTGCATGTATGTATATCAGGGATTTGCGTGTGTGTTTACCT
CTCTTTTTTTTTTGTTAGGTTCCAGCTGCATCATTGTTCAATAATCTGTACAGACGTCAC
AAACGGTAATAATTTGAATTAGATTTTTGATTTTGGTGAAGAATAGTAGCAAAGGACGGA
TTTTGATACTTAGGTTGTAATTATAAATCTGGGTTTGATATGTATGTGTTCTTAGTTCAA
ATTCACTGATTTGTTAATACCTACGACTATTGGTTTATTTGATATTATTCTCCATTTGTT
TGAGTTCCATAGATGACAACTTTCTCTAGGATTTACACAAGTCCTGGCATGTAAAGATGT
AATATTTATGCAATTTCCTTGCAGTTTTGTTCATTGGACGAGTAAGATGTCAACAGATAG
TGTTAGATCATCCACAACTGGGGGTTCTGCTTCTGGAGCTAGAACATTTTGCTCCCTCGC
AGGTATAAATTAGTCATTCCGCTGCTCTACCATTTGATATTTG
   
Sequence Name 22-K026485-022-648-F10C-AG01
GenBank Accession LN495961 [GenBank]
Sequencing primer gene specific primer, AG01: 5'-CTGCCTCAATAAACACAAGTACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K026485-022-648-F10C-AG01
AGCATGTCATGATTCAGTCAAAACAAGGAATTCTTGATTCAAATATCAAATGGTAGAGCA
GCGGAATGACTAATTTATACCTGCGAGGGAGCAAAATGTTCTAGCTCCAGAAGCAGAACC
ACCAGTTGTGGATGATCTAACACTATCTGTTGACATCTTACTCGTCCAATGAACAAAACT
GCAAGGAAATTGCATAAATATTACATCTTTACATGCCAGGACTTGTGTAAATCCTAGAGA
AAGTTGTCATCTATGGAACTCAAACAAATGGAGAATAATATCAAATAAACCAATAGTCGT
AGGTATTAACAAATCAGTGAATCTGAACTAAGAACACATACATATCAAACCCAGATTTAT
AATTACAACCTAAGTATCAAAATCCGTCCTTTGCTACTATTCTTCACCAAAATCAAAAAT
CTAATTCAAATTATTACCGTTTGTGACGTCTGTACAGATTATTGAACAATGATGCAGCTG
GAACCTAACAAAAAAAAAAGAGAGGTAAACACACACGCAAATCCCTGATATACATACTTG
CACATAAACATACTCAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA

Last Updated on 06.12.2018 16:11