Sequences from confirmation amplicon


Confirmation sequences for line 649A07 and hit At5g01630 are shown.
Line ID 649A07
Locus At5g01630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:240595
Primers used for PCR 08im - 8474, show primer details
   
Sequence Name 19-K112877-0022-649-A07C-8409
GenBank Accession LN494834 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112877-0022-649-A07C-8409
CTTTCATTGAATATATCCTGTAGCAATTGAAGCCATTTACATAGTGGAATACATTCTGTA
GAACATGTAGTTCTTAAAGAGTCTTCGATTGCTAAAGCCAAATCCATTTTAGCAGAGAAT
GTTGCTTATTCAGGTATTTTATGTGACAGTATTGAATTCCCTTGACTAGTCATTTGTTTC
TGAATTCGCTTGAATTGTTACTTAATGTTGGTGAATATGTGTTCCTGAAAATCTTTTGAA
TGTTCCATGTTCTTGGCTACACTTTTAGATTTGCAGAACACAAATTGCTCAATTCCCCAA
ACGAGACAAGTGGATACAGCTGAAACTATGCCAATGTTCAGGACGGCCTTGGGGAAAACT
GTCCCTTTGAAAGAGTCTTCAATTGCCAAACCTTTGTCTATTCTAGGAAGTGATATGATA
ATTGACTCAGGTGTGTTCTTATCTCCTCTTTTTTTAAGTCTTATATCCNGCTGTGTGCGC
ATTTACATATGAACTTGTTGTTTGTGCTGCTACTCCGTTCAACTTCCAGGTTTCTTGAGT
   
Sequence Name 56-K112878-0022-649-A07C-08im
GenBank Accession LN509155 [GenBank]
Sequencing primer gene specific primer, 08im: 5'-AACTCAAGAAACCTGGAAGTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K112878-0022-649-A07C-08im
TGTAAATGCGCACACAGCTGGATATAAGACTTAAAAAAAGAGGAGATAAGAACACACNCT
GTAGTCAATTATCATATCACTTCCTAGAATAGACAAAGGTTTGGCAATTGAAGACTCTTT
CAAAGGGACAGTTTTCCCCAAGGCCGTCCTGAACATTGGCATAGTTTCAGCTGTATCCAC
TTGTCTCGTTTGGGGAATTGAGCAATTTGTGTTCTGCAAATCTAAAAGTGTAGCCAAGAA
CATGGAACATTCAAAAGATTTTCAGGAACACATATTCACCAACATTAAGTAACAATTCAA
GCGAATTCAGAAACAAATGACTAGTCAAGGGAATTCAATACTGTCACATAAAATACCTGA
ATAAGCAACATTCTCTGCTAAAATGGATTTGGCTTTAGCAATCGAAGACTCTTTAAGAAC
TACATGTTCTACAGAATGTATTCCACTATGTAAATGGCTTCAATTGCTACAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CACACGGTTATT

Last Updated on 06.12.2018 16:11