Sequences from confirmation amplicon


Confirmation sequences for line 649C02 and hit At3g11330 are shown.
Line ID 649C02
Locus At3g11330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3554749
Primers used for PCR 35fm - 8474, show primer details
   
Sequence Name 25-K111392-0022-649-C02C-35fm
GenBank Accession LN497503 [GenBank]
Sequencing primer gene specific primer, 35fm: 5'-TATCCCAACAACCTCCTCATTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K111392-0022-649-C02C-35fm
CCTCGCCGCATTCTTCTCAGCAGATTCATAAATCCTAACAAGCCTCTCCTCTGCTTCTTT
CAAAAGCTTCTCATACGAAGCATGAACCTCATCAAGCTTCAAAATCGACTCACACCAAGT
CTTCTCCTGATCCATCTCTTGTCTCCTCTTCTCATCTTTCGCCGCCGCATCGGTTAACGC
GATATCCTCGAACGATTCAGATAAGAACGATTCGATTTCCGAGAGTTTAGCACGAGCCTT
ATCGACTGATTCGTGATCTGGTCTAGGTCCTAGGGTTCGAAGGATAGATCGAGTCTCGGC
GACGTCAGAGATCGCACTTGTCATTGAAGCGAGAACATCTGGTTGTGTTAAGTGAGGCAT
CTGAGTGACGATCTCAATCGATGATGATGATGATGATGGTGGTGGTTGTTCGATGTCGAA
GGGAGGAACGGAGGAGGAAGGGGATTTGGCGGTGAAAGAAGGGAGACGAGCCAAGACATA
AGAGAGGACAGGGAAATTCTTAGGGTTAGGTTCTGCCGCCATTGTTTTCGTTAAGCTTCG
GAGATTTGGAATCTGAATCTGGAAATTGATGAAGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATATAGATGTCCGCACGGTTAT
   
Sequence Name 09-K111391-0022-649-C02C-8409
GenBank Accession LN490240 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K111391-0022-649-C02C-8409
ATTTACATTGATATATCTTCATCAATTTCCAGATTCAGATTCCAAATCTCCGAAGCTTAA
CGAAAACAATGGCGGCAGAACCTAACCCTAAGAATTTCCCTGTCCTCTCTTATGTCTTGG
CTCGTCTCCCTTCTTTCACCGCCAAATCCCCTTCCTCCTCCGTTCCTCCCTTCGACATCG
AACAACCACCACCATCATCATCATCATCATCGATTGAGATCGTCACTCAGATGCCTCACT
TAACACAACCAGATGTTCTCGCTTCAATGACAAGTGCGATCTCTGACGTCGCCGAGACTC
GATCTATCCTTCGAACCCTAGGACCTAGACCAGATCACGAATCAGTCGATAAGGCTCGTG
CTAAACTCTCGGAAATCGAATCGTTCTTATCTGAATCGTTCGAGGATATCGCGTTAACCG
ATGCGGCGGCGAAAGATGAGAAGAGGAGACAAGAGATGGATCAGGAGAAGACTTGGTGTG
AGTCGATTTTGAAGCTTGATGAGGTTCATGCTTCGTATGAGAAGCTTTTGAAAGAAGCAG
AGGAGAGGCTTGTTAGGATTTATGAATCTGCTGAGAAGAATGCGGCGGAGGATGAGGAGA
ATGTTGCGGCTGTGGAGGTTAATGAGGAGGTGNTTGGGAT

Last Updated on 06.12.2018 16:11