Sequences from confirmation amplicon


Confirmation sequences for line 649E11 and hit At5g24010 are shown.
Line ID 649E11
Locus At5g24010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8114937
Primers used for PCR 11cl - 8474, show primer details
   
Sequence Name 65-K108072-0022-649-E11C-11cl
GenBank Accession LN511416 [GenBank]
Sequencing primer gene specific primer, 11cl: 5'-AAAGTTATTGGTTCCGGATTGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K108072-0022-649-E11C-11cl
GTAGCCACTAGAACTACAGTTCTTTCAGTTGTTCTACTATTGGAACTGCCTCTAAACCTC
CTCAAAGGTGTCCATCCCGTGCTCTCTGAACTCCTCGTCTTGTTGTTCTTGCGCCTGCAC
AAGCACAAAACGCTCAAGAAGAACAGCGACAAGAACACAAAACCTCCCAAAACCGAGCCA
ACCACGATCCAGACAACATTCCTCTTTCCAGATACCACTTCAGAACTCACAGGGCTCAAA
ATCCTCATGATCTCAACTCCATTCAATAGAGCATTAACTCTCGCCGGATTACTAAGATCA
GACGGTCCAACACTAATCCTCAACATTCCAGAACGATCAGACTCCGCAACAAAATCAATG
TACAACGGAGACGCAAGCACATGGAAAGTAAATGGCTTCCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 49-K108071-0022-649-E11C-8409
GenBank Accession LN506863 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K108071-0022-649-E11C-8409
TTTCTTTCCATGTGCTTGCGTCTCCGTTGTACATTGATTTTGTTGCGGAGTCTGATCGTT
CTGGAATGTTGAGGATTAGTGTTGGACCGTCTGATCTTAGTAATCCGGCGAGAGTTAATG
CTCTATTGAATGGAGTTGAGATCATGAGGATTTTGAGCCCTGTGAGTTCTGAAGTGGTAT
CTGGAAAGAGGAATGTTGTCTGGATCGTGGTTGGCTCGGTTTTGGGAGGTTTTGTGTTCT
TGTCGCTGTTCTTCTTGAGCGTTTTGTGCTTGTGCAGGCGCAAGAACAACAAGACGAGGA
GTTCAGAGAGCACGGGATGGACACCTTTGAGGAGGTTTAGAGGCAGTTCCAATAGTAGAA
CAACTGAAAGAACTGTTAGTTCTAGTGGCTACCATACTCTGAGAATCTCGTTCGCGGAGT
TACAATCCGGAACCAATAACTT

Last Updated on 06.12.2018 16:11