Sequences from confirmation amplicon


Confirmation sequences for line 650D10 and hit At3g53370 are shown.
Line ID 650D10
Locus At3g53370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19788487
Primers used for PCR BA03 - 8474, show primer details
   
Sequence Name 23-K104847-0022-650-D10C-8409
GenBank Accession LN496545 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104847-0022-650-D10C-8409
GCCTTTACATTGTATGGCAATGTTGGCGATTTTTGTTGGGGAAATCGCTTATTGCAAATG
ATTATTTTTTTCTATTCCTCTAAAAAGCTAAGACATCACATAATTTCCTTAGATCATTAA
CAAAATAGCCCACAAATCACGAATCCAACCCTTAAGAAATCGATTGCACATTCCGGGATC
GCAGAAGAGGAGCTATTATGGTAAAATCATCTCGATTTCAACGGAGATACCGTTAAATTC
TACAAAAGGTGAAAGTATAGAGAAGATTGACGGTACCTTTCCGGCAAAATCTTCGCCGTC
CATGATTACGATCCAAAATATACGTGAGATCAGCTCCAGTAACGTACTAATGGTAGGAAG
AGAGGAGGAAAGGAAACGAGGATCTGTTTTCGACACAGAGATTAATAAGACGCAAACAAA
CTCACACAGAGATAATATACACACGTGTTTTATCCACTACATCGCCACTTTCACTAATCC
ATAAATATTATTTTGGACCCCTTACTTTTTAGGTATTTCGATTTCAATCC
   
Sequence Name 08-K104846-0022-650-D10C-BA03
GenBank Accession LN489720 [GenBank]
Sequencing primer gene specific primer, BA03: 5'-CGGATTGAAATCGAAATACCTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104846-0022-650-D10C-BA03
TATATTATGGATAGTGAAGTGGCGATGTAGTGGATAAAACACGTGTGTATATTATCTCTG
TGTGAGTTTGTTTGCGTCTTATTAATCTCTGTGTCGAAAACAGATCCTCGTTTCCTTTCC
TCCTCTCTTCCTACCATTAGTACGTTACTGGAGCTGATCTCACGTATATTTTGGATCGTA
ATCATGGACGGCGAAGATTTTGCCGGAAAGGTACCGTCAATCTTCTCTATACTTTCACCT
TTTGTAGAATTTAACGGTATCTCCGTTGAAATCGAGATGATTTTACCATAATAGCTCCTC
TTCTGCGATCCCGGAATGTGCAATCGATTTCTTAAGGGTTGGATTCGTGATTTGTGGGCT
ATTTTGTTAATGATCTAAGGAAATTATGTGATGTCTTAGCTTTTTAGAGGAATAGAAAAA
AATAATCATTTGCAATAAGCAATTTCCCCAACAAAAATCGCCAACATTGCCATACAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTA

Last Updated on 06.12.2018 16:11