Sequences from confirmation amplicon


Confirmation sequences for line 650F02 and hit At1g21680 are shown.
Line ID 650F02
Locus At1g21680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7613850
Primers used for PCR CQ54 - 8474, show primer details
   
Sequence Name 39-K105634-0022-650-F02C-CQ54
GenBank Accession LN503354 [GenBank]
Sequencing primer gene specific primer, CQ54: 5'-CATCTCATATACGTCACCGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K105634-0022-650-F02C-CQ54
GGCGGTAATTCTGATTTTAAACGAAACGAAGATCGGTGTTAGAAGCACCGTCTCGAGTCC
AAGTTCCGTTGTTATCCCGCTTTGATCATCTGAGTGGCATGACGGTAAATTCTTTTAAAG
ATAAGCCTAGTTTGAGTGGTGAGTTTATTGTCTACGTGTCAACCCATGAGAGCTCAGGTG
AGCCGAGAGCAAGCTGGACCGCCGTTTACTCTACCGAGTTAAAAACCGGGTTAACCAGGC
GGTTAACACCGAGTGGTGTAGCTGATTTCAGCCCGGCGGTATCGCCTTCCGGAAATTTAA
CGGCTGTGGCTTCATACGGAGAGAGAGGCTGGACAGGGGAAGTTGAAGAGCTACGTACGG
ACATCTATGTATTCTTGACTCGGGATGGGTCTCACCGTGTCAAGGTGGTGGAGCACGGTG
GTTGGCCGTGTTGGGTTGATGAGTCGACTCTGTATTGTTTTCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 55-K105635-0022-650-F02C-8409
GenBank Accession LN508812 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K105635-0022-650-F02C-8409
GCCTTTACAATTGAATATATCCTGAAAACAATACAGAGTCGACTCATCAACCCAACACGG
CCAACCACCGTGCTCCACCACCTTGACACGGTGAGACCCATCCCGAGTCAAGAATACATA
GATGTCCGTACGTAGCTCTTCAACTTCCCCTGTCCAGCCTCTCTCTCCGTATGAAGCCAC
AGCCGTTAAATTTCCGGAAGGCGATACCGCCGGGCTGAAATCAGCTACACCACTCGGTGT
TAACCGCCTGGTTAACCCGGTTTTTAACTCGGTAGAGTAAACGGCGGTCCAGCTTGCTCT
CGGCTCACCTGAGCTCTCATGGGTTGACACGTAGACAATAAACTCACCACTCAAACTAGG
CTTATCTTTAAAAGAATTTACCGTCATGCCACTCAGATGATCAAAGCGGGATAACAACGG
AACTTGGACTCGAGACGGTGCTTCTAACACCGATCTTCGTTTCGTTTTAAAATCAGAATT
ACCGCCGTAAACTAGATCGTAGTACAAGCTAGAAGTACCGTTTCTCTCGGTGAC

Last Updated on 06.12.2018 16:11