Sequences from confirmation amplicon


Confirmation sequences for line 650F10 and hit At5g26170 are shown.
Line ID 650F10
Locus At5g26170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9148171
Primers used for PCR IS53 - 8474, show primer details
   
Sequence Name 07-K022566-022-650-F10C-8409
GenBank Accession LN489076 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K022566-022-650-F10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATAGTGGATCACGCATTCTTATAGGC
GTAATTCGTAAATAGTGGTGAGTATGAATGATGCAGACACAAACTTGGGGAGTAGTTTCA
GCGATGATACTCACTCTGTGTTCGAGTTTCCGGAGCTAGACTTGTCAGATGAATGGATGG
ATGATGATCTTGTGTCTGCGGTTTCCGGGATGAATCAGTCTTATGGTTATCAGAATAGTG
ATGTTGCTGGGGCTTTATTC
   
Sequence Name 08-K022566-022-650-F10C-IS53
GenBank Accession LN489543 [GenBank]
Sequencing primer gene specific primer, IS53: 5'-GGGCTTATGTTTTTGAATTAACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K022566-022-650-F10C-IS53
TGATCTGTTCTTATTGTTAGAAACCAAAAGAGAAACTTACCGGCAGAAGCAGTGGCTGTA
GCAGCAACATAAGTTTTGGTACTTGGAGATTCAGGATGACTGAAACAGCTAGAAGAACCT
GAGAATAAAGCACCAGCAACATCACTAGTCTGATAACCATAAGACTGATTCATCCCGGAA
ACCGCAGACACAAGATCATCATCCATCCATTCATCTGACAAGTCTAGCTCCGGAAACTCG
AACACAGAGTGAGTATCATCGCTGAAACTACTCCCCAAGTTTGTGTCTGCATCATTCATA
CTCACCACTATTTACGAATTACGCCTATAAGAATGCGTGATCCACTATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11