Sequences from confirmation amplicon


Confirmation sequences for line 651C03 and hit At1g62200 are shown.
Line ID 651C03
Locus At1g62200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22983881
Primers used for PCR 36op - 8474, show primer details
   
Sequence Name 37-K114909-0022-651-C03C-36op
GenBank Accession LN502720 [GenBank]
Sequencing primer gene specific primer, 36op: 5'-TAAATGAACCAATGTTGATGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K114909-0022-651-C03C-36op
TCGTTTCCTCGGGTCAGTATCATTAAACTGATTCAGCACCAAAAGAAGATACACATGGGT
TTGATACCTCCAGTACCAAGAGCAATAAGGTAAAGCCCTGTAAAGAAAACCGCATACTGA
ACCGTTGTTGCTGGTGAGCATAGAGCCGCAGCAACGCCAGCACATGCCGCTGGCTTTAAA
ACCGGAAGTGAAGCCGAGAGTGTCAACAAAGCCATTCCCTAAATTCAAAAACCAAGATTA
ACTAACTATGCAAATCACCTAAACAAACAAATGCAAGTATCCAAAGAGATTGATTAGTCT
TACAATGAAATAAATAGCAGAGAAAGAAGCAATGGTCCAGTATCTTCCCCAGTAAGAATC
AGCTATAACAGCTCCAATGAGAGGTGTGATATAGCAAGTTCCTTGCCAAATCATAACGTC
ACTAGCTGCAGAAACATTGGACTCGTGTAATTCACTTGTGTAGTAAGTGATTAGATTCTT
GGCAATTCCATAGTAAGCTAAAACAATTCCATAAAAAAATTCTTGTTTTATCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAATGTAATT
   
Sequence Name 13-K114908-0022-651-C03C-8409
GenBank Accession LN492156 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114908-0022-651-C03C-8409
TTTTTTTACGGAATCGTTTTAGCTTACTATGGAATTGCCAAGAATCTAATCACTTACTAC
ACAAGTGAATTACACGAGTTCAATGTTTCTGCAGCTAGTGACGTTATGATTTGGCAAGGA
ACTTGCTATATCACACCTCTCATTGGAGCTGTTATAGCTGATTCTTACTGGGGAAGATAC
TGGACCATTGGTTTCTTTCTCTGCTATTTATTTCATTGTAAGACTAATCAATCTCTTTGG
ATACTTGCATTTGTTTGTTTAGGTGATTTGCATAGTTAGTTAATCTTGGTTTTTGAATTT
AGGGAATGGCTTTGTTGACACTCTCGGCTTCACTTCCGGTTTTAAAGCCAGCGGCATGTG
CTGGCGTTGCTGCGGCTCTATGCTCACCAGCAACAACGGTTCAGTATGCGGTTTTCTTTA
CAGGGCTTTACCTTATTGCTCTTGGTACTGGAGGTATCAAACCATGTGTATCATCTTTTG
GTGCTGATCAGTTTGATGATACTGACCCGAGGGAACGAGTTAGAAAAGCTTCTTTCTTTA
ACTGGTTTTACTTCTCCAT

Last Updated on 06.12.2018 16:11