Sequences from confirmation amplicon


Confirmation sequences for line 651C08 and hit At1g18200 are shown.
Line ID 651C08
Locus At1g18200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6265727
Primers used for PCR NA52 - 8474, show primer details
   
Sequence Name 52-K101565-0022-651-C08C-8409
GenBank Accession LN507764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101565-0022-651-C08C-8409
TTTACATTGAATATGATGTTACCGACGAGAACACGACAGTCTCGGGACTGGAGAAGCCTC
TGAGCTCCGACAACCATTTCTCAATGTTTTTGAAAGTGATTCGTCTTGTGATGTCGTAGA
TTAGTAAAGCTCCCAAGGCTCCACGGTAATACGAACTCGTTATTGCTCTAAATCTTTTTT
CCTCCCATGCAAAAATAAAAAATAAAACAGTATTATATTTAAGATAAGTAAAAACACATA
AATTATTAAAGAAATTCACAATTCTAAATGAAAGATAAAATTTGTCGAAATAAATATAAT
TTTAGTAAGAAAAACAAAAAGAAGAAATGGTCGTGACATTTAACAAAGATTAGGTCCGTG
TGACATCGATTGCTTATAAAGATTTTCTTCTGCGCGTGATCTATTTTCCTAATCATCATT
CCAATCCCAAATTTCATTCATTATTTTTTTGTTATTTATCTAGCAAATTTACCTTAACAT
TTAGACACAAAAATAACTATCGAAAATTTAACAAGTTTGACGTCTTGGTTTG
   
Sequence Name 52-K101578-0022-651-C08C-NA52
GenBank Accession LN507765 [GenBank]
Sequencing primer gene specific primer, NA52: 5'-CAAACCAAGACGTCAAACTTGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101578-0022-651-C08C-NA52
GGTAATATGCTAGATAAATAACAAAAAAATAATGAATGAAATTTGGGATTGGAATGATGA
TTAGGAAAATAGATCACGCGCAGAAGAAAATCTTTATAAGCAATCGATGTCACACGGACC
TAATCTTTGTTAAATGTCACGACCATTTCTTCTTTTTGTTTTTCTTACTAAAATTATATT
TATTTCGACAAATTTTATCTTTCATTTAGAATTGTGAATTTCTTTAATAATTTATGTGTT
TTTACTTATCTTAAATATAATACTGTTTTATTTTTTATTTTTGCATGGGAGGAAAAAAGA
TTTAGAGCAATAACGAGTTCGTATTACCGTGGAGCCTTGGGAGCTTTACTAATCTACGAC
ATCACAAGACGAATCACTTTCAAAAACATTGAGAAATGGTTGTCGGAGCTCAGAGGCTTC
TCCAGTCCCGAGACTGTCGTTGTTCTCGTCGGTNACATCATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11