Sequences from confirmation amplicon


Confirmation sequences for line 651C10 and hit At3g09530 are shown.
Line ID 651C10
Locus At3g09530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2926332
Primers used for PCR DN42 - 8474, show primer details
   
Sequence Name 26-K101829-0022-651-C10C-8409
GenBank Accession LN497863 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K101829-0022-651-C10C-8409
ATTTATGGTAATTGTTTAGTCTACTTTTTTTATATATCCATATAGTCTAATGTATGGTAG
AAGAAACTAAAACCGCTCTTGCTCCTCATCTTTGATCTTACGTCTCTGTTTTTTTTTTTT
AAATTAAGGTCGTTGCAGGAATTTTGAAGGAAAAATCAAAGGGTTTTAAGGGTAAAATTT
ACATTTAATATTGAAACGCCCCGGCTTGTATGTGGATTCAAAAACTAGGGGTGAAAAGGG
TTCCCTTTTAAAAAACCTACCACTTAATCCAAATGCAGGGAGGCGTCTTTACGTTAAAAC
AAAAACGGTAAATATTTTTAATCTGTTACGCAACCATAAGGAAAAATTTAGGTCGTTTAT
CGTTATGGTTACAAAACAACGTCAGCAAAATATGGGGAAAAATGTGCCGTTGGCCTTTGA
CTCACCTTTTCCGTACA
   
Sequence Name 70-K101832-0022-651-C10C-DN42
GenBank Accession LN512356 [GenBank]
Sequencing primer gene specific primer, DN42: 5'-AAGAGACACGTGTACGGAAAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101832-0022-651-C10C-DN42
ATATTTCGCTGACGTTGTTTTGTAACCATAACGATAAACGACCTAAATCTTTCATTATGG
TTGCGTAACAGATTAAAAATATCTACAGTTTCTGTTCTAACGTAACGACGCCTCCCTGCA
TTTGGATTAAGTGGTAGGTTCTTTAAAAGGGAACCCTTTTCACCACTAGTTCTTGAATCC
ACATACAAGCCGAGGCGTTTCAATATTAAATGTAAATTTTACCCTTAAAACCATTTGATT
TCTCCTTCAAAATTCCTGCAACGACATTAATTTGAAAAAAAAAAAACAGAGACTTAAAAT
CAAAGATGAGGAGCAAGAGCGGTTTTAGTTTCTTCTACCATACATTAAACTATATGGATA
TATAAAAAAAGTAAACTAAACAATTACCATAAATGGCTTCATGTCCGGGAAATCTACATG
GATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATTTCC

Last Updated on 06.12.2018 16:11