Sequences from confirmation amplicon


Confirmation sequences for line 651D08 and hit At3g62770 are shown.
Line ID 651D08
Locus At3g62770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23220392
Primers used for PCR JL33 - 8474, show primer details
   
Sequence Name 24-K032518-022-651-D08C-8409
GenBank Accession LN496890 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K032518-022-651-D08C-8409
CGGACAATTCGTCATGGCTCAGATTCCAGAATAGCTTGCTTCGCTCTCACGCAGGATGGC
CATTTGTTGGCCACTGCTAGCTCTAAGGGTACTCTGGTTCGGATCTTCAATACTGTTGAT
GGTACCTTGCGTCAAGAGGTACGCTTTTTATTCACAGAAAGCTGAATACTTTCTAATGAT
TCATGTAAGAATGCTGGCAAATTTGGTTGTTTAGTCTCTGACTTGTAGTCTGGCACTTCT
GAGGATGAAATAGGTAAAAGAAAGAAAGTAGGACTATATTTCTATTATTAGCTTAGAGAT
TCAGAAAAGTGCTCTTTTATATCCTCGTGATTG
   
Sequence Name 24-K032518-022-651-D08C-JL33
GenBank Accession LN496891 [GenBank]
Sequencing primer gene specific primer, JL33: 5'-GCAATCACGAGGATATAAAAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K032518-022-651-D08C-JL33
ATATAGAATATAGTCCTACTTTCTTTCTTTTACCTATTTCATCCTCAGAAGTGCCAGACT
ACAAGTCAGAGACTAAACAACCAAATTTGCCAGCATTCTTACATGAATCATTAGAAAGTA
TTCAGCTTTCTGTGAATAAAAAGCGTACCTCTTGACGCAAGGTACCATCAACAGTATTGA
AGATCCGAACCAGAGTACCCTTAGAGCTAGCAGTGGCCAACAAATGGCCATCCTGCGTGA
GAGCGAAGCAAGCTATTCTGGAATCATGAGCCATGACGAATTTGGTCCGGGAAATCTACA
TGGTTCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAAATGGGA
Primers used for PCR FQ48 - 3144, show primer details
   
Sequence Name 37-K033039-022-651-D08C-3144
GenBank Accession LN502517 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K033039-022-651-D08C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTCCCGACGTGAATGTAGACA
CGTCGAAATAAAGATTTCCGAATTAGAATAATTTGTTTATTGCTTTCGCCTATAAATACG
ACGGATCGTAATTTGTCGTTTTATCAAAATGTACTTTCATTTTATAATAACGCTGCGGAC
ATCTACATTTTTGAATTGAAAAAAAATTGGTAATTACTCTTTCTTTTTCTCCATATTGAC
CATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAAGTACATAACTTTCGTT
TAGAAGCGTAGTGCTCGATCCGAACTTGACCTTTCTGCAAACCTGGACATACCAAAACCA
TAGAACCAACACCCTGAGAAACAGCACACAAACCCTTAGGGTTGGCAATGGTTTCAATCT
GATGCATCAGCTTGAGGTCAGAGAAATTGTAGACAAAAATCTTCTGCTCAAGAACGACAA
TAATCCGATCCCTCCTAAGCCGGACGGATCTAACATCGGACCTGAAAGAGAGTTCTCCGA
TACATCGGCCCTGGTGATCATCCCAAATCATAACCTTATTAGGAGGATATTGAGGATCAG
GTCCGCCACCAACTAGGGCTAATATATTGCATCTGAAAAGCATCTCCACGACTGCAACAC
CACCGCCACGATCGAAATCACGCCGGAAAATCTCGCGAAAGGGATCGCAATTAAGGATCC
GGAAGCCACGGTCAGTGCCGACAGCGAAGCAAGCATGATCTTGGTTAAAGGAAAGATGAA
GCACGGACGGAGGAGGCATCACCGGTAAATTGGGCGTANGGGGAGAAAGAG
   
Sequence Name 05-K033039-022-651-D08C-FQ48
GenBank Accession LN488244 [GenBank]
Sequencing primer gene specific primer, FQ48: 5'-TCAGACTTCTAATCAATCGCCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K033039-022-651-D08C-FQ48
ATGCCTCCTCCGTCCGTGCTTCATCTTTCCTTTAACCAGATCATGCTTGCTTCGCTGTCG
GCACTGACCGTGGCTTCCGGATCCTTAATTGCGATCCCTTTCGCGAGATTTTCCGGCGTG
ATTTCGATCGTGGCGGTGGTGTTGCAGTCGTGGAGATGCTTTTCAGATGCAATATATTAG
CCCTAGTTGGTGGCGGACCTGATCCTCAATATCCTCCTAATAAGGTTATGATTTGGGATG
ATCACCAGGGCCGATGTATCGGAGAACTCTCTTTCAGGTCCGATGTTAGATCCGTCCGGC
TTAGGAGGGATCGGATTATTGTCGTTCTTGAGCAGAAGATTTTTGTCTACAATTTCTCTG
ACCTCAAGCTGATGCATCAGATTGAAACCATTGCCAACCCTAAGGGTTTGTGTGCTGTTT
CTCAGGGTGTTGGTTCTATGGTTTTGGTATGTCCAGGTTTGCAGAAAGGTCAAGTTCGGA
TCGAGCACTACGCTTCTAAACGAAAGTTATGTACTTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCACGTCGGGAAACCGAAGGCGGGAAACGACAA
TCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGG
GTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATNC
ATCCACTTGCTTTGAAGACGTGGTTGGAACGT

Last Updated on 06.12.2018 16:11