Sequences from confirmation amplicon


Confirmation sequences for line 651E11 and hit At5g56390 are shown.
Line ID 651E11
Locus At5g56390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22842949
Primers used for PCR 86rq - 8474, show primer details
   
Sequence Name 64-K116204-0022-651-E11C-86rq
GenBank Accession LN511275 [GenBank]
Sequencing primer gene specific primer, 86rq: 5'-AAACTAAGGAGATTTTGGGCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K116204-0022-651-E11C-86rq
CTACACTTCAAGGTACATGTTGTTTAGTACACATTATTTGTTTTCTCTTTTGGAAGGATA
AAAATAAACTGTCACTAATCGAGTTCTCTTATGATCAAAACTAAATTATGCAGGATGCGT
ATCCTGTTGGTAATATATTTGGCTCTCTTATACATTTAACTATATGCACATGTGAGACAG
AATGGTTGAATGTACTTATACGTGTGCTCAGAGATTCCCCTAATTTAAAAACACTCAAAA
TTGAACAGGTATGCACATATATTTCCCTTTAATGCATTAAGTGTTTTAGATATGGTTATT
TTTACCAGTACGATTGATATGATGCGATGTACTTGTATCTCAGTATCATAACCTTCGGGA
TGAAGACCCACGCCCGTGCTGGAACGAAACGAGCTTGGTGCCTGAATATCTGTTACCTAG
TCTTGAAACCTTTGAATGGGTAGATTATGAAGGAACAAAAACAGAGAAACAAGTCGTAGC
ATTCATCTTGAGAATTGCAATGAGTATCCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K116203-0022-651-E11C-8409
GenBank Accession LN498994 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116203-0022-651-E11C-8409
GACTTGTTTCTCTGTTTTTGTTCCTTCATAATCTACCCATTCAAAGGTTTCAAGACTAGG
TAACAGATATTCAGGCACCAAGCTCGTTTCGTTCCAGCACGGGCGTGGGTCTTCATCCCG
AAGGTTATGATACTGAGATACAAGTACATCGCATCATATCAATCGTACTGGTAAAAATAA
CCATATCTAAAACACTTAATGCATTAAAGGGAAATATATGTGCATACCTGTTCAATTTTG
AGTGTTTTTAAATTAGGGGAATCTCTGAGCACACGTATAAGTACATTCAACCATTCTGTC
TCACATGTGCATATAGTTAAATGTATAAGAGAGCCAAATATATTACCAACAGGATACGCA
TCCTGCATAATTTAGTTTTGATCATAAGAGAACTCGATTAGTGACAGTTTATTTTTATCC
TTCCAAAAGAGAAAACAAATAATGTGTACTAAACAACATGTTACCTTTGAAGTTGGTAAG
CAGAGAAAAAGACGTTTGGTTGAAGTTAGAGAGCCCA

Last Updated on 06.12.2018 16:11