Sequences from confirmation amplicon


Confirmation sequences for line 652F12 and hit At3g02230 are shown.
Line ID 652F12
Locus At3g02230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:416228
Primers used for PCR AC12 - 8474, show primer details
   
Sequence Name 31-K027789-022-652-F12C-8409
GenBank Accession LN500020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K027789-022-652-F12C-8409
CATTTACATTGAATATGCATAAAGAAAGCCCCACAAATAAAGTTTGGACTTTTCTTTGCC
TTAAGCCTTATGACCACAACATAAATGTGCAGGACAATGACATGCAAGAATCTTGTGAAG
TTAACTGTTAAGACATAACATAGAAAGGTTGGTCCAACAACAATGACATTACCTAAACAT
AAGAGAGATCATGGCAATGCCGACAAACATAAGACTAACACAATTTCAATTCTGAAAACA
GATCGAAACCCATCAAGCAGATGAATATATAGATCTAAGTCAAAACAGTGTGTTTCTTAA
AGTTGACCTAGAGAATTGTGATCGTATTTTCAATTTTCAATTCAAAGATCAGTCAAATCT
ACAGACTTTATCACTAGATCTCAAACTGAACCTATGAAGAAGGAAAAAAAGAACAGATCT
ATAAACAGAATTGTGGAAACTTACGAAGCAATC
   
Sequence Name 32-K027789-022-652-F12C-AC12
GenBank Accession LN500441 [GenBank]
Sequencing primer gene specific primer, AC12: 5'-TTCACTATTGATGACGATTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K027789-022-652-F12C-AC12
GATCTGTTCTTTTTTTCCTTCTTCATAGGTTCAGTTTGAGATCTAGTGATAAAGTCTGTA
GATTTGACTGATCTTTGAATTGAAAATTGAAAATACGATCACAATTCTCTAGGTCAACTT
TAAGAAACACACTGTTTTGACTTAGATCTATATATTCATCTGCTTGATGGGTTTCGATCT
GTTTTCAGAATTGAAATTGTGTTAGTCTTATGTTTGTCGGCATTGCCATGATCTCTCTTA
TGTTTAGGTAATGTCATTGTTGTTGGACCAACCTTTCTATGTTATGTCTTAACAGTTAAC
TTCACAAGATTCTTGCATGTCATTGTCCTGCACATTTATGTTGTGGTCATAAGGCTTAAG
GCAAAGAAAAGTCCAAACTTTATTTGTGGGGCTTTCTTTATGCATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11