Sequences from confirmation amplicon


Confirmation sequences for line 654A03 and hit At2g41400 are shown.
Line ID 654A03
Locus At2g41400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17259542
Primers used for PCR SR45 - 8474, show primer details
   
Sequence Name 52-K103544-0022-654-A03C-SR45
GenBank Accession LN507786 [GenBank]
Sequencing primer gene specific primer, SR45: 5'-TACATCATTGTCGAGAAGATCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103544-0022-654-A03C-SR45
GGCTGAGAATGTTGTGGCGGCGTAGCTAGCGATAACAATGAAATTCTCGAGGCTGATGCT
ACCCCCATATATTTCATTAACTACTGAGGCTATGAGTACGCCGCCCGGTGGATAGATAAA
GCATGAGTTAGTCGGAAGATTTACTTTAATGTCGCACTTACTTGGATTATCAAAACTCGT
GTTGGCGGGATTGAACACAAGGGTAAATACACCAACCTGATTGGTCACTGCTTGAGCGAG
GGTTGAGTTTGAGCCTGGACATTCAATATAGACTGTGGCATTGGAGACAGGTGGTGCGCT
TGGATCTCCGTCAAGGCTACAATACACGACACCACGAATGCTGACTCGGTCGATTGTGTA
GGACCAGAGTTTAAAGGCGTGGGATATGTGCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 68-K103545-0022-654-A03C-8409
GenBank Accession LN511993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K103545-0022-654-A03C-8409
TCGGACATGAGCCTTTACAATTGAATATATCCTGCACATATCCCACGCCTTTAAACTCTG
GTCCTACACAATCGACCGAGTCAGCATTCGTGGTGTCGTGTATTGTAGCCTTGACGGAGA
TCCAAGCGCACCACCTGTCTCCAATGCCACAGTCTATATTGAATGTCCAGGCTCAAACTC
AACCCTCGCTCAAGCAGTGACCAATCAGGTTGGTGTATTTACCCTTGTGTTCAATCCCGC
CAACACGAGTTTTGATAATCCAAGTAAGTGCGACATTAAAGTAAATCTTCCGACTAACTC
ATGCTTTATCTATCCACCGGGCGGCGTACTCATAGCCTCAGTAGTTAATGAAATATATGG
GGGTAGCATCAGCCTCGAGAATTTCATTGTTATCGCTAGCTACGCCGCCACAACATTTCT
CAGCCCTGGTTAATGTTTATCGCTAACTATGCCATCGGTGATCTTCTCGACAATGATGT
Primers used for PCR SR46 - 8474, show primer details
   
Sequence Name 15-K103729-0022-654-A03C-SR46
GenBank Accession LN492940 [GenBank]
Sequencing primer gene specific primer, SR46: 5'-CACGTACACTAGCTACTGCGCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103729-0022-654-A03C-SR46
ACACATTCACATTAACTAAACTTCAATGTGATCAAAGGAAACACTTTTAAAACAAATACA
TATTTCTATTTCCTTTTCCTTGGAGCATTTTCATGCATTCAAAGCTTTTGCTTGTGAATC
ATATATACTATATTCCTATACATATATATATCTCGTCGTGCAAGTAAGGATCACAATTAA
ATACATTACTTCAGTAAACGTACACTTCCTAAAAGGATTTTGGCAAGCAAACACAACTAT
CATCGAATTTTGGAATTAATTTTGCCAGTCCATGATTTATCCAATTAGCAAATCAAATAT
CTCATACATATACTCATATAAATACTACTCCCCTTACGCTTTATTTGCCACACAACACCT
TCAGTTAAAACATCTTTAAAACGAAATCAAAACAGCTGATAACCAAAAAAATAAAAAAAA
TATGGAAAAATCTAATGTTTTCCTCCTCTCCATACATTACAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 31-K103730-0022-654-A03C-8409
GenBank Accession LN500131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103730-0022-654-A03C-8409
GGACTGAGCCTTTACAATTGTAATGTATGGAGAGGAGGAAAACATTAGATTTTTCCATAT
TTTTTTTATTTTTTTGGTTATCAGCTGTTTTGATTTCGTTTTAAAGATGTTTTAACTGAA
GGTGTTGTGTGGCAAATAAAGCGTAAGGGGAGTAGTATTTATATGAGTATATGTATGAGA
TATTTGATTTGCTAATTGGATAAATCATGGACTGGCAAAATTAATTCCAAAATTCGATGA
TAGTTGTGTTTGCTTGCCAAAATCCTTTTAGGAAGTGTACGTTTACTGAAGTAATGTATT
TAATTGTGATCCTTACTTGCACGACGAGATATATATATGTATAGGAATATAGTATATATG
ATTCACAAGCAAAAGCTTTGAATGCATGAAAATGCTCCAAGGAAAAGGAAATAGAAATAT
GTATTTGTTTTAAAAGTGTTTCCTTTGATCACATTTGAAGTTGTAGTTAATGTGAATGTG
TTCTAGTGAAACAAGAATAGATGGTGTAGCGCAGTAGCAGTGGTACGTG

Last Updated on 06.12.2018 16:11