Sequences from confirmation amplicon


Confirmation sequences for line 654G08 and hit At4g37260 are shown.
Line ID 654G08
Locus At4g37260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17540590
Primers used for PCR MF33 - 8474, show primer details
   
Sequence Name 64-K033096-022-654-G08C-8409
GenBank Accession LN511129 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K033096-022-654-G08C-8409
TCCGATAAAGATCCGGCGATGTCAACCCGACCCGTAAGAATATGGAGAGGATTAAAGGTC
CATGGAGTCCAGAAGAAGATGATCTGTTGCAGAGGCTTGTTCAGAAACATGGTCCGAGGA
ACTGGTCTTTGATTAGCAAATCAATCCCTGGACGTTCCGGCAAATCTTGTCGTCTCCGGT
GGTGTAACCAGCTATCTCCGGAGGTAGAGCACCGTGCTTTTTCGCAGGAAGAAGACGAGA
CGATTATTCGAGCTCACGCTCGGTTTGGTAACAAGTGGGCTACGATCTCTCGTCTTCTCA
ATGGACGAACCGATAACGCTATCAAGAATCATTGGAACTCGACGCTGAAGCGAAAATGCA
GCGTCGAAGGGCAAAGTTGTGATTTTGGTGGTAATGGAGGGTATGATGGTAATTTAGGAG
AAGAGCAACCGTTGAAACGTACGGCGAGTGGTGGTGGTGGTGTCTCGACTGGCTTGTATA
TGAGTCCCGGAAGTCCATCGGGATCTGACGTCAGCGAGCAATCTAGTGGTGGTGCACACG
TGTTTAAACCAACGGTTAGATCTGA
   
Sequence Name 16-K033096-022-654-G08C-MF33
GenBank Accession LN493334 [GenBank]
Sequencing primer gene specific primer, MF33: 5'-GACGCTGTAACCTCAGATCTAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K033096-022-654-G08C-MF33
ACTAGATTGCTCGCTGACGTCAGATCCCGATGGACTTCCGGGACTCATATACAAGCCAGT
CGAGACACCACCACCACCACTCGCCGTACGTTTCAACGGTTGCTCTTCTCCTAAATTACC
ATCATACCCTCCATTACCACCAAAATCACAACTTTGCCCTTCGACGCTGCATTTTCGCTT
CAGCGTCGAGTTCCAATGATTCTTGATAGCGTTATCGGTTCGTCCATTGAGAAGACGAGA
GATCGTAGCCCACTTGTTACCAAACCGAGCGTGAGCTCGAATAATCGTCTCGTCTTCTTC
CTGCGAAAAAGCACGGTGCTCTACCTCCGGAGATAGCTGGTTACACCACCGGAGACGACA
AGATTTGCCGGAACGTCCAGGGATTGATTTGCTAATCAAAGACCAGTTCCTCGGACCATG
TTTCTGAACAAGCCTCTGCAACAGATCATCTTCTTCTGGACTCCATGGACCTTTAATCCT
CTCCATATTCTTACGGGTCGGGTTTGACATCGCCGGATCTTTTTATCGGATTGTTCTTTT
TTTTTCTTCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTC

Last Updated on 06.12.2018 16:11