Sequences from confirmation amplicon


Confirmation sequences for line 655A03 and hit At4g22010 are shown.
Line ID 655A03
Locus At4g22010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11664041
Primers used for PCR BW12 - 8474, show primer details
   
Sequence Name 12-K104268-0022-655-A03C-BW12
GenBank Accession LN491557 [GenBank]
Sequencing primer gene specific primer, BW12: 5'-TTCACCTGGAAAATTACTTATGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104268-0022-655-A03C-BW12
GTATTTCAACTGTTTCTTCTTCTTTCTTTCAGGTTTGTCACATTTTCTCTGTTTGTTCTC
TCGAGAAATCTTGTGATAACATTATCTTGGGTTGGGTTTTGAGTACTGCAGGGAATATTG
ATAAATGGGCAGTTTCCTGGACCTCACATTGACGCAATCACAAATGATAATATCATTATC
AGTGTTTTCAATTACTTGAAAGAGCCTTTTCTCATTTCTTGGTAAGTTCTTTCACATATC
TTTACTTTTTTTGCCGTATTATGTGTTGCCGAGAAGTTGAATATTCTTGGATAGAAAGCA
ATAAAGATTGAAGATTAGGATAAGATCTTAACTTTTAATTTTTGTGAATTTTGTTTTTGT
TTTTTGTTGTCCAAAAGAATGCAGCTTTCGGACAAATGTGCTTTGAGTAAATCTCATCTC
ACACATTATCAATACTTGCATATTTATGTATTAGTCTACAAGAGAAAGAAATGATACGCA
ATGGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCACGGTTATT
   
Sequence Name 28-K104269-0022-655-A03C-8409
GenBank Accession LN498824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104269-0022-655-A03C-8409
TTACATTGATCCATTGCGTATCATTTCTTTCTCTTGTAGACTAATACATAAATATGCAAG
TATTGATAATGTGTGAGATGAGATTTACTCAAAGCACATTTGTCCGAAAGCTGCATTCTT
TTGGACAACAAAAAACAAAAACAAAATTCACAAAAATTAAAAGTTAAGATCTTATCCTAA
TCTTCAATCTTTATTGCTTTCTATCCAAGAATATTCAACTTCTCGGCAACACATAATACG
GCAAAAAAAGTAAAGATATGTGAAAGAACTTACCAAGAAATGAGAAAAGGCTCTTTCAAG
TAATTGAAAACACTGATAATGATATTATCATTTGTGATTGCGTCAATGTGAGGTCCAGGA
AACTGCCCATTTATCAATATTCCCTGCAGTACTCAAAACCCAACCCAAGATAATGTTATC
ACAAGATTTCTCGAGAGAACAAACAGAGAAAATGTGACAAACCTAGAAAGAAAGAAGAAG
AAACAGTTGAAATACCTGTTGTTTAACACCAAGGGGATAAATGTCGCCATAAGTAATTTT
CCAGGTGA

Last Updated on 06.12.2018 16:11