Sequences from confirmation amplicon


Confirmation sequences for line 655B06 and hit At5g45900 are shown.
Line ID 655B06
Locus At5g45900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18617236
Primers used for PCR JL34 - 8474, show primer details
   
Sequence Name 23-K032518-022-655-B06C-8409
GenBank Accession LN496449 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K032518-022-655-B06C-8409
GACCTAGAAATCTTCAAATTATAGAGAGATTGCCTGACTGGATTAGACATGGCAACTTTT
CCATAGTCAACAAAGGTGATATTGCGGATTCCCCAACCCTAAGAAAATAAAAGATAAACG
AGTTCTTAGTTAATCAGTGAGTTTGTAAAATAATGTCAAATCTCGCATTTTCTTCAATAC
GTACCATAAGGGTACGAGCAACTTGGCAACCCAATGTACCAGCTCCAAGAAGAAGGCATT
TGACAGAGGACAATACATTTAAGTTAAGAGACGGTAATGCTCGCCATCTCATTAACTTTA
AGTTTAAATCGACAGCAGAAACAGCCAACCTGCGAAAGAACGTGATTCCGTTTGTCTAAT
CAGATTTTACCCATTTAGTTGAGTAGATCATTGACACAGAGCAAAAATGTACCTTGTTGG
ATCCATGGAATTAGCAAGGCTAATGGATCGGGGTACACGTTTCCCTTTGTTAAGCTCCCA
TCCCACTGAGTTAGGTACAGTCTCAGCTGATTCACCTGATGAAAGTG
   
Sequence Name 23-K032518-022-655-B06C-JL34
GenBank Accession LN496450 [GenBank]
Sequencing primer gene specific primer, JL34: 5'-CAAGCCTCAATTACACTTTCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K032518-022-655-B06C-JL34
GGGATGGGAGCTTAACAAAGGGAAACGTGTACCCCGATCCATTAGCCTTGCTAATTCCAT
GGATCCAACAAGGTACATTTTTGCTCTGTGTCAATGATCTACTCAACTAAATGGGTAAAA
TCTGATTAGACAAACGGAATCACGTTCTTTCGCAGGTTGGCTGTTTCTGCTGTCGATTTA
AACTTAAAGTTAATGAGATGGCGAGCATTACCGTCTCTTAACTTAAATGTATTGTCCTCT
GTCAAATGCCTTCTTCTTGGAGCTGGTACATTGGGTTGCCAAGTTGCTCGTACCCTTATG
GTACGTATTGAAGAAAATGCGAGATTTGACATTATTTTACAAACTCACTGATTAACTAAG
AACTCGTTTATCTTTTATTTTCTTAGGGTTGGGGAATCCGCAATATCACCTTTGTTGACT
ATGGAAAAGTTGCCATGTCTAATCCAGTCAGGCAATCTCTCTATAATTTTGAAGATTGTC
TAGGTCGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACT
Primers used for PCR FQ49 - 8474, show primer details
   
Sequence Name 59-K118414-0022-655-B06C-FQ49
GenBank Accession LN510105 [GenBank]
Sequencing primer gene specific primer, FQ49: 5'-AAATTCACATCAAACACCGACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118414-0022-655-B06C-FQ49
AAGAATAAGAAACGAGTTAGTTACCTTATTGGCATTAGCACAAAGAAGAGACGGTAACCA
CCTGCTTTCTCTTGTATCGGTTAACAAGAACACCGCATCATGAGATTCAATCAACTCGCT
AAGGCGTTTACAATCACCGAGAACAGAATCTTCTTCTTGGCTAGAAATCGGATGTCCGGG
CATCGGTATAGCCATAACAACTCCACTAGTTTCCATTGCTGGAAATATCTGTTTAAGGCT
TTTAATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACCGATTATTATAAA
   
Sequence Name 11-K118413-0022-655-B06C-8409
GenBank Accession LN491327 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118413-0022-655-B06C-8409
TTGAATATATCCTGCAATTAAAAGCCTTAAACAGATATTTCCAGCAATGGAAACTAGTGG
AGTTGTTATGGCTATACCGATGCCCGGACATCCGATTTCTAGCCAAGAAGAAGATTCTGT
TCTCGGTGATTGTAAACGCCTTAGCGAGTTGATTGAATCTCATGATGCGGTGTTCTTGTT
AACCGATACAAGAGAAAGCAGGTGGTTACCGTCTCTTCTTTGTGCTAATGCCAATAAGGT
AACTAACTCTGTTTCTTTATTTCTTCTTACTGGTTATATAAGTATAACAATCTGCTTGAT
ATGTCGGTGTTTGATGTGAATTT

Last Updated on 06.12.2018 16:11