Sequences from confirmation amplicon


Confirmation sequences for line 655C01 and hit At1g11730 are shown.
Line ID 655C01
Locus At1g11730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3959720
Primers used for PCR 43sa - 8474, show primer details
   
Sequence Name 62-K116229-0022-655-C01C-43sa
GenBank Accession LN510834 [GenBank]
Sequencing primer gene specific primer, 43sa: 5'-TATCATCCATCCATTTTGTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K116229-0022-655-C01C-43sa
GGCTTTATCCGCACACCAAAACAAGCCTCGGGTTTATGTTGGTTGCATGAAGTCTGGTCC
CGTCCTAGCCAGAAAGTAAGTAAAAACCCTACTAAGGTGAGCATTTTTTAGGTACTTAAA
CAGCGTAAGAGCATCAACCTTTCCGGACTTTCTATACTTCTGGCAAAAAAAAAAACAGGA
GTGTGAAGTACCATGACCCAAATTATTGAAATTTTGAAAAATTCGGGAACAATTATTTCC
CCCACGCCACTGGACAGTTTTATGCTATATCTAAAGATCTAGCTACTTACATACGGATAA
ACCAGTAAGTTTTTTTCCAAACTTGGTTTTACCCAGCGGTTTAATGCACCCAAAAGGCTG
GGAAAAAACCTCTAACAATCTCTATTTATTTTGTTGAAATAAGGAATTTACTGCATAATT
ATGCAAATGAAAATGTTTCGTTGGAATCTGGGTTCATCGAATTGAATGTAAAACATGTTG
ATAAAAAAAAACTTTGTTGCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAA
TATGGAGAAAAAAAAAAAGTAATTACCATTTTTTTTTCATTTCAAAAATGTA
   
Sequence Name 22-K116228-0022-655-C01C-8409
GenBank Accession LN496265 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116228-0022-655-C01C-8409
AACGAAACATTCTTTCATTCGCATACTTATGCAGTAAATCCCCTTATTTTCAACAAAATA
AATAGAGATCGTTAGAGGTTTTTTCCCAAGCATTCTGGCTGCATTAAACCGCTGGCTAAA
ACCAAGTTAGGAAAAAAACTTACTGGTTTATCAGTATGTAAGTAGCTAGATCTTTAGATA
TAGCATAAAACTGTCCAGTGGCGTGGCGGAAATACTTGTTCCCGACTTCTCCAAACTTCC
AATACTCTGGCTCATGGTACTTCACACTCCTGTTTTTTTTTTTGCCAAAATTATAAAAAG
TCCGGAAAGGTTGATGCTCTTACCCTGTTTAATTACCTAAAAAATGCTCACCTTAGTAGG
GTTTTTACTTACTTTCTGGCTAGAACGGGACCAAACTTCATGCAACCAACATAAACCCAA
GGTTTGTTTTGGTGTGCGAATAAACCCTTCTTGAGGGAGGCTGCAAAAAATCGAATCAGG
GCTTCAAAA

Last Updated on 06.12.2018 16:11