Sequences from confirmation amplicon


Confirmation sequences for line 655D01 and hit At1g02910 are shown.
Line ID 655D01
Locus At1g02910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:656589
Primers used for PCR CQ55 - 8474, show primer details
   
Sequence Name 36-K107033-0022-655-D01C-8409
GenBank Accession LN502242 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K107033-0022-655-D01C-8409
GCCATTTTTGACTGTGAGTTTCTTTTTCTACTTTTTACTGCCTGGAGTGTGAATTAGCCT
TTGAGTTTCTGAGTAGTGATTGACACTGTCGTTAGGTTATTCTAGCTGGGAAAAAAGAGA
CTGTTACACTTGCGATGCAAAAAGCAGATAGGTTCAGAACCGAGCTCCTAAGACGAGGTG
TTCTCTTAGTTCCTGTGGTATGGGGTGAACGCAAAACACCGGAAATCGAGAAAAAAGGTT
TTGGAGCTTCTTCAAAGGCAGCTACATCTCTTCCTTCAATTGGGGTAACGAAAAGATTCT
GTCTTTGAAAAAAACAAACAAAATATTCTCATTTTGCAAAACAGTGATGTTCTCTTAACT
TGATAGGAAGATTTTGATACACGGGCTCAATCGGTTGTAGCCCAATCGAAGCTGAAAGGT
GAAATCAGGTTCAAGGCCGAGACTGTTTCGCCTGGTGAATGGGAAAGGTCGAAACTTTCA
TTGCCATTTGTTGCTGATCAAAGTTTGATAGAAGAAAGATGGCAACTTTGTTGTTGAGTC
TTAAAGATGTCTCTGGTTTTGTTTGTTTCAGGTGGATAAGAGATCAACAGATATCTGAAG
GAGTTAACCCGGGCGATGATGTGTACATCATACTGCGGTTAGATGGTCGAGTCCGCAGAT
CGGGTAGAGTAAGTTTCATCTAGAAACCTTTTCCTCTCTTGCTTTCTCGTGTTTGATATC
TACTTATTGGTTTT
   
Sequence Name 44-K107034-0022-655-D01C-CQ55
GenBank Accession LN505199 [GenBank]
Sequencing primer gene specific primer, CQ55: 5'-CAAAACCTCCTCAAAAACCAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107034-0022-655-D01C-CQ55
CAGAGAGGAAAGGTTTCTAGATGAAACTTACTCTACCCGATCTGCGGACTCGACCATCTA
ACCGCAGTATGATGTACACATCATCGCCCGGGTTAACTCCTTCAGATATCTGTTGATCTC
TTATCCACCTGAAACAAACAAAACCAGAGACATCTTTAAGACTCAACAACAAAGTTGCCA
TCTTTCTTCTATCAAACTTTGATCAGCAACAAATGGCAATGAAAGTTTCGACCTTTCCCA
TTCACCAGGCGAAACAGTCTCGGCCTTGAACCTGATTTCACCTTTCAGCTTCGATTGGGC
TACAACCGATTGAGCCCGTGTATCAAAATCTTCCTATCAAGTTAAGAGAACATCACTGTT
TTGCAAAATGAGAATATTTTGTTTGTTTTTTTCAAAGACAGAATCTTTTCGTTACCCCAA
TTGAAGGAAGAGATGTAGCTGCCTTTGAAGAAGCTCCAAAACCTTTTTTCTCGATTTCCG
GTGTTTTGCGTTCACCCCATACCACAGGAACTAAGAGAACACCTCGTCTTAGGAGCTCGG
TTCTGAACCTATCTGCTTTTTGCATCGCAAGTGTAACAGTCTCTTTTTTCCCAGCTAGAA
TAACCTAACGACAGTGTCAATCACTACTCAGAAACTCAAAGGCTAATTCACACTCCAGGC
AGTAAAAAGTAGAAAAAGAAACTCACAGTCAAAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11