Sequences from confirmation amplicon


Confirmation sequences for line 655D02 and hit At3g12990 are shown.
Line ID 655D02
Locus At3g12990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4157625
Primers used for PCR LK01 - 8474, show primer details
   
Sequence Name 43-K027640-022-655-D02C-8409
GenBank Accession LN504717 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K027640-022-655-D02C-8409
CATTTCATTGAATATATCCTGCAACAACAAAGATAATTAGAGATGCAGTAGGGTTTCGTA
ATCCGGAGCGTTTTCCTTTTTAGATTGATTTCTATTGATTTAAGGATTTGATTTTGTTGT
GCTTTGTTTTCATGGTGTAGGTTGAAGCATACAACCGTGAGAGGAGCTCACAGAAGGTGA
AGCGGCATCATACTTTGGCTAAGTCTGAAGTTTTGGGACCTATTGTAGTTGTGTAGGAAG
AGACATAAATAAAACATTTGCTGTGGTCGTCGATTGTAAATAAAGATTTTAGTTGTAGAA
ATCTTATCAGCAACACTAAGCTATAAAAACGTCGATATGATCAAGACATGTTGAGACTTC
TCGCACTTAAATAGATGACTAGTCTCAACTCTTCCATTTATGTCTTTCTGAAAGAAAGTT
GAAACACAACATATTGAGACTTCTCAGAATTAATATGCATATCCTGAATAACATGTACAC
TATAAGCCAAAAGCTATGTTGGAATAATAGTAGTAACCATGATCATGAAACAGCCTCCTC
TCACTGTTCGTTCTTATGAGGTCGGTTAAAGCTTTTTTTACAACAGAAGAGAGTCTTTGA
ACTGTTATTACAACGAGGTGTTGTCGTCGGGGAGAAA
   
Sequence Name 44-K027640-022-655-D02C-LK01
GenBank Accession LN505060 [GenBank]
Sequencing primer gene specific primer, LK01: 5'-CTCGTTTCAGATACCTGTTTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K027640-022-655-D02C-LK01
ATACAGTTCAAGACTCTCTTCTGTTGTAAAAAAGCTTTAACCGACCTCATAAGAACGAAC
AGTGAGAGGAGGCTGTTTCATGATCATGGTTACTACTATTATTCCAACATAGCTTTTGGC
TTATAGTGTACATGTTATTCAGGATATGCATATTAATTCTGAGAAGTCTCAATATGTTGT
GTTTCAACTTTCTTTCAGAAAGACATAAATGGAAGAGTTGAGACTAGTCATCTATTTAAG
TGCGAGAAGTCTCAACATGTCTTGATCATATCGACGTTTTTATAGCTTAGTGTTGCTGAT
AAGATTTCTACAACTAAAATCTTTATTTACAATCGACGACCACAGCAAATGTTTTATTTA
TGTCTCTTCCTACACAACTACAATAGGTCCCAAAACTTCAGACTTAGCCAAAGTATGATG
CCGCTTCACCTTCTGTGAGCTCCTCTCACGGTTGTATGCTTCAACCTACACCATGAAAAC
AAAGCACAACAAAATCAAATCCTTAAATCAATAGAAATCAATCTAAAAAGGAAAACGCTC
CGGATTACGAAACCCTACTGCATCTCTNATTATCTTTGTTGTTGCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11