Sequences from confirmation amplicon


Confirmation sequences for line 655E04 and hit At3g25780 are shown.
Line ID 655E04
Locus At3g25780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9410229
Primers used for PCR 23eq - 8474, show primer details
   
Sequence Name 23-K110827-0022-655-E04C-23eq
GenBank Accession LN496606 [GenBank]
Sequencing primer gene specific primer, 23eq: 5'-AACGAAGGAGATAGAAACAGTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K110827-0022-655-E04C-23eq
AGCTTTGTCTCGGAGATCTCGTCCCATTCACAAACAAACTCTACACCGGCGATCTCAAGA
AACGCGTGGGAATCACGGCAGGTCTCTGCGTTTTGATCCAACACGTTCCTGAAAAGAGCG
GTGACAGATTCGAAGCTAGTTACAGCTTCTACTTTGGTGACTATGGTCACTTGTCTGTAC
AAGGACAATACTTGACTTATGAAGACACGTTCCTCGCTGTCACTGGTGGCTCTGGAATCT
TTGAAGGTGCGTACGGACAGGTTCAATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 07-K110826-0022-655-E04C-8409
GenBank Accession LN489328 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K110826-0022-655-E04C-8409
GCCATTTACATTGAATATATTGAACCTGTCCGTACGCACCTTCAAAGATTCCAGAGCCAC
CAGTGACAGCGAGGAACGTGTCTTCATAAGTCAAGTATTGTCCTTGTACAGACAAGTGAC
CATAGTCACCAAAGTAGAAGCTGTAACTAGCTTCGAATCTGTCACCGCTCTTTTCAGGAA
CGTGTTGGATCAAAACGCAGAGACCTGCCGTGATTCCCACGCGTTTCTTGAGATCGCCGG
TGTAGAGTTTGTTTGTGAATGGGACGAGATCTCCGAGACAAAGCTCTGTTGGTTTCTTGC
CGAGTTTAAGAACTGCTGGACTGTTTCTATCTCCTTCGT

Last Updated on 06.12.2018 16:11