Sequences from confirmation amplicon


Confirmation sequences for line 656C01 and hit At5g24910 are shown.
Line ID 656C01
Locus At5g24910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8569110
Primers used for PCR HM55 - 8474, show primer details
   
Sequence Name 55-K101220-0022-656-C01C-8409
GenBank Accession LN508750 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101220-0022-656-C01C-8409
TTTACATTGTTAAGTTCTTTCACCAACTCCGGGTGGTTCATGTACAAGTGTTGCTTCACC
CCCGTCGAATACGTGTACACTCTCCCTGAACATATCAATTTTTTTCAATAAATTACTCAT
ACAAAATAAATATACAATAAATGCTCAAAACCAACTTAGAAAACGGTGAAAATCAAAAAC
TTAAGATTTCCTCTCAATCAATATGACCGATCATAAACTATTAAACGATCCACTAGCGAA
GCGACAACTACTTTGGTATTTTAAAACTAAATTTAAAATATATCTTACGATTTATTACTA
TAAAAAAAAGGGAATCAAAATGAATCATTGAGGAGTTATCATATAATTGATCAGCTCCTA
ACTTTTGGATCAAGTAGACCCGATCGTACAAAACCTTATTGGCTTTTCATAAATTTTAAA
TTCTGCGGCGTTAGTTGTCATTAGGTGTCA
   
Sequence Name 55-K101227-0022-656-C01C-HM55
GenBank Accession LN508751 [GenBank]
Sequencing primer gene specific primer, HM55: 5'-ATGGATTGACATGACACCTAATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101227-0022-656-C01C-HM55
ATTATGAAAGCCATAAGGTTTTGTACGATCGGGTCTACTTGATCCAAAGTTAGGAGCTGA
TCAATTATATGATAACTCCTCAATGATTCATTTTGATTCCCTTTTTTTTATAGTAATAAA
TCGTAAGATATATTTTAAATTTAGTTTTAAAATACCAAAGTAGTTGTCGCTTCGCTAGTG
GATCGTTTAATAGTTTATGATCGGTCATATTGATTGAGAGGAAATCTTAAGTTTTTGATT
TTCACCGTTTTCTAAGTTGGTTTTGAGCATTTATTGTATATTTATTTTGTATGAGTAATT
TATTGAAAAAAATTGATATGTTCAGGGAGAGTGTACACGTATTCGACGGGGGTGAAGCAA
CACTTGTACATGAACCACCCGGAGTTGGTGAAAGAACTTAAACAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11