Sequences from confirmation amplicon


Confirmation sequences for line 658D03 and hit At1g18100 are shown.
Line ID 658D03
Locus At1g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6228910
Primers used for PCR DM13 - 8474, show primer details
   
Sequence Name 81-K101748-0022-658-D03C-8409
GenBank Accession LN514047 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K101748-0022-658-D03C-8409
ATTTACATTGAATAATCCTGATGGGCATAATTAACTATTACTATATTTTCTTAATGCCTT
TCACACTAACGTCACTTTATTTATAAATTATAATGCATTAATATACAAAGCAATAAATCT
GTTTACCGTCGTCAAAAAATATAGTAAAGAGCCAAAGACAATCCTAAAGTAAAGTCAAGT
AATTGTATTCAAAATCGAAAACTTAGCTTTAATCTTTAGTATTCAATTCATACCTCTTGA
GGGATTTGTGCCTCCGGGAATATCCACGACAATCCTGAAAATAAGAAATATAATTAATAG
TGTTTTATTGCACCTTTATTTATACGTTAAATTGTTTACGTTGGTGTANAATTGCTT
   
Sequence Name 81-K101754-0022-658-D03C-DM13
GenBank Accession LN514048 [GenBank]
Sequencing primer gene specific primer, DM13: 5'-GAAGCAATTGTACACCAACGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K101754-0022-658-D03C-DM13
TAGGTGCATAAACACTATTATTATATTTCTTATTTTCAGGATTGTCGTGGATATTCCCGG
AGGCACAAATCCCTCAAGAGGTATGAATTGAATACTAAAGATTAAAGCTAAGTTTTCGAT
TTTGAATACAATTACTTGACTTTACTTTAGGATTGTCTTTGGCTCTTTACTATATTTTTT
GACGACGGTAAACAGATTTATTGCTTTGTATATTAATGCATTATAATTTATAAATAAAGT
GACGTTAGTGTGAAAGGCATTAAGAAAATATAGTAATAGTTAATTATGCCCATCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGNTTATT

Last Updated on 06.12.2018 16:11