Sequences from confirmation amplicon


Confirmation sequences for line 658E04 and hit At5g13560 are shown.
Line ID 658E04
Locus At5g13560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4362069
Primers used for PCR 35uj - 8474, show primer details
   
Sequence Name 35-K117356-0022-658-E04C-35uj
GenBank Accession LN501983 [GenBank]
Sequencing primer gene specific primer, 35uj: 5'-CAGATCAGGGAGACAATCAGGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117356-0022-658-E04C-35uj
ACTATGAGACCAGGTAAGAATTGCTGCTTAGTAGGTTACGATTGATTATTTTTATTGAGA
CCAAATATGACTCTGCATGGTCGTGCTCTGGTTCGTTGATTTTTTTTCTTTTTCTGGTTT
CTATTCTTCCTCGTCTCTTATTTGGTTTTATCAAAAACTTAGATCATTACCACATTCAGC
ATTGTGGACAACGTGAAAGAGCAATTCCAGGTTCTTAGTCATGACCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCG
AGATTATTAT
   
Sequence Name 15-K117355-0022-658-E04C-8409
GenBank Accession LN493152 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117355-0022-658-E04C-8409
GATATATCCTGGTCATGACTAAGAACCTGGAATTGCTCTTTCACGTTGTCCACAATGCTG
AATGTGGTAATGATCTAAGTTTTTGATAAAACCAAATAAGAGACGAGGAAGAATAGAAAC
CAGAAAAAGAAAAAAAATCAACGAACCAGAGCACGACCATGCAGAGTCATATTTGGTCTC
AATAAAAATAATCAATCGTAACCTACTAAGCAGCAATTCTTACCTTGGTCTCATAGTCTA
TATACTCCTCCTCCAGAATTTTCCTCGGGTTTGATACCTGATTGTCTCCCTGATCTG

Last Updated on 06.12.2018 16:11