Sequences from confirmation amplicon


Confirmation sequences for line 658H04 and hit At2g14510 are shown.
Line ID 658H04
Locus At2g14510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6173181
Primers used for PCR EU34 - 8474, show primer details
   
Sequence Name 06-K103431-0022-658-H04C-EU34
GenBank Accession LN488773 [GenBank]
Sequencing primer gene specific primer, EU34: 5'-AACCAACACGATTATAGTAACGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103431-0022-658-H04C-EU34
GCACATCGCCACCGCCAGGATTTTGGTTGGTGTTTCCCGCGCCGCGTTATATTTGGATCA
ACACTAAAAAATGAAAACGTTATTGACGAAATATTAACTATATAAAAATGTGTCGATAAT
AATCAAAACTAGATCATCGAAAAACAGACTCTTACAATAGTTTTAAACCATCATTCTTTT
CTCTATCTTGAAGGGCTTGAGGAACAGAACCTCTAAGATTGTTTCCTCTTAAGTGTCTGT
GGATGTGGAGAAATCATTAGAAACTAATTAATGAAGACAACACAATAATATTTAGGTAAC
TTACATGACCAACAACGGTTTGATAGTGGCTAGAAATTCAGGCACTTCTCCAGTCAAGTT
GTTATTTGATAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCACGGTTATT
   
Sequence Name 22-K103432-0022-658-H04C-8409
GenBank Accession LN496076 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K103432-0022-658-H04C-8409
GCCATTTACTTTATCAAATAACAACTTGACTGGAGAAGTGCCTGAATTTCTAGCCACTAT
CAAACCGTTGTTGGTCATGTAAGTTACCTAAATATTATTGTGTTGTCTTCATTAATTAGT
TTCTAATGATTTCTCCACATCCACAGACACTTAAGAGGAAACAATCTTAGAGGTTCTGTT
CCTCAAGCCCTTCAAGATAGAGAAAAGAATGATGGTTTAAAACTATTGTAAGAGTCTGTT
TTTCGATGATCTAGTTTTGATTATTATCGACACATTTTTATATAGTTAATATTTCGTCAA
TAACGTTTTCATTTTTTAGTGTTGATCCAAATATAACGCGGCGCGGGAAACACCAACCAA
AATCCTGGCTGGTGGCGATTGTTGCATCTATATCTTGTGTGGCCGTTACTATAATCGTGT
TGGT

Last Updated on 06.12.2018 16:11