Sequences from confirmation amplicon


Confirmation sequences for line 659H05 and hit At5g59100 are shown.
Line ID 659H05
Locus At5g59100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23859547
Primers used for PCR 47uw - 8474, show primer details
   
Sequence Name 27-K118269-0022-659-H05C-47uw
GenBank Accession LN498593 [GenBank]
Sequencing primer gene specific primer, 47uw: 5'-AGTTATGTTGAACGGTTTTGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118269-0022-659-H05C-47uw
TTTCTTGGTAATGTCTTGGAAATTTCTTTCGTGCAAGTGCTGTTGTCTCCGGAGATGATT
CTTAAGTGATCACTCGTGTAGTTTAAACCACAGAGAAAGTTGATGTGGTCTGCTTTGGTC
AATTCATAAACGAGTCCAGGATTAATAGCATCTATCGGGTCCACATGGCCAGATCCATAT
GCAAACTCAGTTGATACAAAGCCAGAACCAGAAGCATTCATTGGCCAAGCTACAAAATTA
GGAGATCATCAATGGGTCTCTTGCGGCACAAGTGAGTTTGTCTTGGACAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
AACGGTTATT
   
Sequence Name 12-K118268-0022-659-H05C-8409
GenBank Accession LN491789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118268-0022-659-H05C-8409
TTACATTGAATATATCCTGTCCAGACAAACTCACTTGTGCCGCAAGAGACCCATTGATGA
TCTCCTAATTTTGTAGCTTGGCCAATGAATGCTTCTGGTTCTGGCTTTGTATCAACTGAG
TTTGCATATGGATCTGGCCATGTGGACCCGATAGATGCTATTAATCCTGGACTCGTTTAT
GAATTGACCAAAGCAGACCACATCAACTTTCTCTGTGGTTTAAACTACACGAGTGATCAC
TTAAGAATCATCTCCGGAGACAACAGCACTTGCACGAAAGAAATTTCCAAGACATTACCA
AGAAACCTCAACTACCCAACAATGTCGGCTAAAGTGTCGGGAACAAAACCGTCAAACATA
AC

Last Updated on 06.12.2018 16:11