Sequences from confirmation amplicon


Confirmation sequences for line 660B08 and hit At1g27260 are shown.
Line ID 660B08
Locus At1g27260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9470901
Primers used for PCR 35ny - 8474, show primer details
   
Sequence Name 42-K114669-0022-660-B08C-35ny
GenBank Accession LN504559 [GenBank]
Sequencing primer gene specific primer, 35ny: 5'-AATTCTTGTTTGGTTTGCTCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114669-0022-660-B08C-35ny
TTTCTCCTCTCAGTTTCTATTAAAAGATGACCCCCACGATAACCAGAAAATATTAAGTGA
AAAAAACATTTTGATATTAAAGAAAGCTCTACAAAAGAAACTATACACTATCTTTTAGTA
TCTTATACACCAATTCATTGAGCATAGAAGCATAGCATATTTGTGAATGTCATGTTAATC
AAAGCTAAACTCAAATGTTTACAAAGAAAACTAAAAACACTCTCTTCTCATTAGAAGCTC
TACTCATATTTTTGTCTCTGAAGGTATAAACAGTCTTCCGGTACCGTCCTTGCCTATCTC
TAGACTCTATGGATCACTGAAAATAATTTTTATAAGCTCCATGACTAAATCTTCATGATC
CTGAACAAGTGGAGAAAAAGAAAGAGTAATTACCAATTTCATGTAGATTTTCTTTGTGTT
TGGTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGAT
   
Sequence Name 19-K114668-0022-660-B08C-8409
GenBank Accession LN494870 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114668-0022-660-B08C-8409
CTGGAACCAACACAAAGAAAATCTACATGAAATTGGTAATTACTCTTTCTTTTTCTCCAC
TTGTTCAGGATCATGAAGATTTAGTCATGGAGCTTATAAAAATTATTTTCAGTGATCCAT
AGAGTCTAGAGATAGGCAAGGACGGTACCGGAAGACTGTTTATACCTTCAGAGACAAAAA
TATGAGTAGAGCTTCTAATGAGAAGAGAGTGTTTTTAGTTTTCTTTGTAAACATTTGAGT
TTAGCTTTGATTAACATGACATTCACAAATATGCTATGCTTCTATGCTCAATGAATTGGT
GTATAAGATACTAAAAGATAGTGTATAGTTTCTTTTGTAGAGCTTTCTTTAATATCAAAA
TGTTTTTTTCACTTAATATTTTCTGGTTATCTTTGGGGTCATCTTTTAATAGAAACTGAG
AGGAGAAACACTCAAAATCAGAGTTCAACGTTGAAACAATAACAGAGAGGAACAGAG

Last Updated on 06.12.2018 16:11