Sequences from confirmation amplicon


Confirmation sequences for line 660D03 and hit At5g04690 are shown.
Line ID 660D03
Locus At5g04690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1350514
Primers used for PCR 45tv - 8474, show primer details
   
Sequence Name 57-K117029-0022-660-D03C-45tv
GenBank Accession LN509525 [GenBank]
Sequencing primer gene specific primer, 45tv: 5'-TATCGAGACAAACAATGTCGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K117029-0022-660-D03C-45tv
CGCGGTGAGTACTCCGAGGAATATGAGGACGGAAGTGCAGGCGGCGAAGCAGGAGATCAG
ATCGGAGACGATGAATATGACGAATATTCGTTCATGTCGGTGAAAGGGTCTACCGCCTGA
GTTGTCGTCCGTGCCGCCGGGGACGGTGAAGATTGCCGCGAAGGTGACCGTGACGATGAG
AGCCGCTACTAAGCTGCAAGACATCGCAGTATCTTTCATCCACTTCTCGGCTTCTAGTCG
CATTGCTTCGTGCTCCTTTCTGAATATCTCGATCGGTGTCAGATTTTCTGTGTTTACTCT
CTCATTTTCGATCGCCGGCACGATTCTCTCCATCTCCTACATTATCAAGACTATATATTT
TTTAGAAATCCGTTTTAGTTATGCATTTAAGGTGCCTACTCCATTAAATCTATAGCAGAG
AGAAACAAAGCGAACCTTAAACCATTGTAACTCGCGTTGCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 25-K117028-0022-660-D03C-8409
GenBank Accession LN497624 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117028-0022-660-D03C-8409
ATCCCTGCAACGCGAGTTACAATGGTTTAAGGTTCGCTTTGTTTCTCTCTGCTATAGATT
TAATGGAGTAGGCACCTTAAATGCATAACTAAAACGGATTTCTAAAAAATATATAGTCTT
GATAATGTAGGAGATGGAGAGAATCGTGCCGGCGATCGAAAATGAGAGAGTAAACACAGA
AAATCTGACACCGATCGAGATATTCAGAAAGGAGCACGAAGCAATGCGACTAGAAGCCGA
GAAGTGGATGAAAGATACTGCGATGTCTTGCAGCTTAGTAGCGGCTCTCATCGTCACGGT
CACCTTCGCGGCAATCTTCACCGTCCCCGGCGGCACGGACGACAACTCAGGCGGTAGACC
CTTTCACCGACATGAACGAATATTCGTCATATTCATCGTCTCCGATCTGATCTCCTGCTT
CGCCGCCTGCACTTCCGTCCTCATATTCCTCGGGATACTCACCGCGAGATACGCTTTCGA
CGATTTCTTGTTCTCGCTGCCTGCGAATATGATCGCTGGACTTTCGACATTG

Last Updated on 06.12.2018 16:11