Sequences from confirmation amplicon


Confirmation sequences for line 660G10 and hit At3g16800 are shown.
Line ID 660G10
Locus At3g16800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5722060
Primers used for PCR 52jx - 8474, show primer details
   
Sequence Name 81-K113379-0022-660-G10C-52jx
GenBank Accession LN514091 [GenBank]
Sequencing primer gene specific primer, 52jx: 5'-CTTTAAGGCAGTAATCTCCAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K113379-0022-660-G10C-52jx
CATACCCACCCGGTACACTCCCGGTTCATCGTCTAGGCAGAACAATCGTCCATCCGATTG
TTTTATCCGTTCTGCTTCCTCTGTTTTAACCGGAAATTATCACATACCAATTGTTAATTC
GTCATTTCATCTGAGTAAAAGTAACTGTAAAATATGGAAAGGCTTCTAGAACTCTACCGG
GAATGTTTGGTTTAAAGTCTACCGAGAGCTGAACCGGGACTAAACCGTTTCCATCATCAG
AAGTTGTTGCTATTACTGCTCGTGAGTCACCCGCATTTGCTATAACGAGATGATCACCCT
TAAAAAGACAAAATCAAACTTTATCATTTAGCATCTTTCAAATGAAACATTATAAATACT
CACAAAATATTATCTATACACCATAAAATGAAAAGTTATAATATGGTTACAAGAACTTAT
GTATTTTTGTTTTTTGCTAAAGGTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 43-K113377-0022-660-G10C-8409
GenBank Accession LN504903 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113377-0022-660-G10C-8409
CATTTCATTGAATATATCCTGAACACCTTTAGCAAAAAACAAAAATACATAAGTTCTTGT
AACCATATTATAACTTTTCATTTTATGGTGTATAGATAATATTTTGTGAGTATTTATAAT
GTTTCATTTGAAAGATGCTAAATGATAAAGTTTGATTTTGTCTTTTTAAGGGTGATCATC
TCGTTATAGCAAATGCGGGTGACTCACGAGCAGTAATAGCAACAACTTCTGATGATGGAA
ACGGTTTAGTCCCGGTTCAGCTCTCGGTAGACTTTAAACCAAACATTCCCGGTAGAGTTC
TAGAAGCCTTTCCATATTTTACAGTTACTTTTACTCAGATGAAATGACGAATTAACAATT
GGTATGTGATAATTTCCGGTTAAAACAGAGGAAGCAGAACGGATAAAACAATCGGATGGA
CGATTGTTCTGCCTAGACGATGAACCGGGAGTGTACCGGGTGGGTATGCCTAATGGAGGA
TCACTCGGTTTAGCTGTTTCAAGAGCGTTTGGAGATTACTGCCTTAAAG

Last Updated on 06.12.2018 16:11