Sequences from confirmation amplicon


Confirmation sequences for line 660H07 and hit At5g49990 are shown.
Line ID 660H07
Locus At5g49990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20340173
Primers used for PCR AC36 - 8474, show primer details
   
Sequence Name 13-K027916-022-660-H07C-8409
GenBank Accession LN491890 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K027916-022-660-H07C-8409
CATTTACAATTGAATTTACGATTTAAGAGGATAATTCGGGCAACCCAAGGTGCTCTTATT
GTTGCTTCAACTCTTCAAATTATCCTTGGCTTCAGTGGCCTTTGGCGTAATGTTGTAAGG
TCAGTCTTTGAGCTACATTAGACTCAAATCTTGTTCCACTGTGTCAGGTTAAAGAATATG
ATATTGAACTTCTGTTCGTCCAGGTTCTTAAGTCCACTATCTGCTGCTCCTTTGGTTGGA
TTAGTTGGTTATGGCCTGTACGAGTTAGGTTTTCCCGGTGTAAGTCTCTTTGTTCTGTAA
ATGGGAACAGATAAACTAAATTATATCAACCTAGTGGAATCTTGATTCTTTGTTATTTTG
GCCAGGTTGCTAAGTGTATTGAGATTGGACTGCCTGGGCTTATTATTCTCATACTAATAT
CACAGGTAATTGTAGGTAAAGATTCATAACTGGTAGCAATTTGCAGCCGGCTCATCTGTT
CTCATTGCATAAGTTATTGATCCGGATGTGTTCTTCCAGTATGTTGATGTTTCTAATCAT
CTGGGTCATACTTTTTGCTGGTTTTGT
   
Sequence Name 14-K027916-022-660-H07C-AC36
GenBank Accession LN492361 [GenBank]
Sequencing primer gene specific primer, AC36: 5'-TAACATGGGGCATGTACTACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K027916-022-660-H07C-AC36
ACATACTGGAAGAACCATCCGGATCAATAACTTATGCAATGAGAACAGATGAGCCGGCTG
CAAATTGCTACCAGTTATGAATCTTTACCTACAATTACCTGTGATATTAGTATGAGAATA
ATAAGCCCAGGCAGTCCAATCTCAATACACTTAGCAACCTGGCCAAAATAACAAAGAATC
AAGATTCCACTAGGTTGATATAATTTAGTTTATCTGTTCCCATTTACAGAACAAAGAGAC
TTACACCGGGAAAACCTAACTCGTACAGGCCATAACCAACTAATCCAACCAAAGGAGCAG
CAGATAGTGGACTTAAGAACCTGGACGAACAGAAGTTCAATATCATATTCTTTAACCTGA
CACAGTGGAACAAGATTTGAGTCTAATGTAGCTCAAAGACTGACCTTACAACATTACGCC
AAAGGCCACTGAAGCCAAGGATAATTTGAAGAGTTGAAGCAACAATAAGAGCACCTTGGG
TTGCCCGAATTATCCTCTTAAATCGTAAATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATT

Last Updated on 06.12.2018 16:11