Sequences from confirmation amplicon


Confirmation sequences for line 661F08 and hit At4g37330 are shown.
Line ID 661F08
Locus At4g37330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17564028
Primers used for PCR NG93 - 8474, show primer details
   
Sequence Name 90-K102372-0022-661-F08C-8409
GenBank Accession LN515031 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K102372-0022-661-F08C-8409
TTACAATTGCATGTTAGTATTCATTACTTTATCTTTGGTTTGTCAACTAACAAATTTATT
ATTTATTCATTACTCGTGACGACTACTTTCATATAGTACATACTTTTATATTTATTGTGG
TGATAAATTGTCCATGTGACTAGTTTATTTTTTTGAAGCATTGCGTGTATATTACGATAC
TAGCTATTTATTCACGAAAGTCTGATCACTATATTCATAAATATTACATAGTTTATTCAC
AAATTACAAATGTACAGAAAAGCTTAATTTTAAATTTATAGAAAAAGATGCAGTGTAGGT
TTTAATTGTCTTAAGTTGGTTGAATAATTATTTGGTTCAAAATAAACACATTGTTCTAAT
ATGTTAAGGTGGCGAATATGGAAATGCACAAATTTTTACATACTTACCAAAACTCTTATC
TGTTTACTAATATTGTATTCGTACTCTCAGGGATTTGTGGAGGTAGAGATGAAAACATTG
TTAACCAATTTGGCATCCAACACCACCATCAGAATGTTGGCCGGGAAGAGATATTTCGGT
GAGGACAACGATGACGCTAAACTCGTGAAGAACCTTGTGTCGGAGGCGGTGACCAGCGCC
GGTGCAGGAAACCCCATTGATTATCTTTCCATTTAGGTTTGGGT
   
Sequence Name 90-K102373-0022-661-F08C-NG93
GenBank Accession LN515032 [GenBank]
Sequencing primer gene specific primer, NG93: 5'-GACCCAACGTAAAATGGAAAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K102373-0022-661-F08C-NG93
GCCTCCGAACAGGTTCTTCCGAGTTTAGCGTCATCGTTGTCCTCACCGAAATATCTCTTC
CCGGCCAACATTCTGATGGTGGTGTTGGATGCCAAATTGGTTAACAATGTTTTCATCTCT
ACCTCCACAAATCCCTGAGAGTACGAATACAATATTAGTAAACAGATAAGAGTTTTGGTA
AGTATGTAAAAATTTGTGCATTTCCATATTCGCCACCTTAACATATTAGAACAATGTGTT
TATTTTGAACCAAATAATTATTCAACCAACTTAAGACAATTAAAACCTACACTGCATCTT
TTTCTATAAATTTAAAATTAAGCTTTTCTGTACATTTGTAATTTGTGAATAAACTATGTA
ATATTTATGAATATAGTGATCAGACTTTCGTGAATAAATAGCTAGTATCGTAATATACAC
GCAATGCTTCAAAAAAATAAACTAGTCACATGGACAATTTATCACCACAATAAATATAAA
AGTATGTACTATATGAAAGTAGTCGTCACGAGTAATGAATAAATAATAAATTTGTTAGTT
GACAAACCAAAGATAAAGTAATGAATACTAACATGCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGAGGGTCAATAT

Last Updated on 06.12.2018 16:11