Sequences from confirmation amplicon


Confirmation sequences for line 663C11 and hit At2g34880 are shown.
Line ID 663C11
Locus At2g34880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14712909
Primers used for PCR BG29 - 8474, show primer details
   
Sequence Name 27-K101744-0022-663-C11C-8409
GenBank Accession LN498322 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K101744-0022-663-C11C-8409
GCCTTTACATTGAATATATCCTGTAGAAAATGAAACGGTCTTTCCCTTCACATGAACAAA
TATCGTGTTTCGTGCAACCATATTCTTCGAGAGATGAGCAATTCTTGCAGCCAGTAGCAG
AGAGATGCAAGTCACTAAAGCAAGAGATGCATTCCCTTTCACAGTTTGAATCAAAGTCTT
TATCCATCTTTACCAAGCTGAAACCATTTCCAAGAGCCTCAATTCTTTTTTCTTCCATCC
GTAACCGTGCCTCGATGGCCTTAGTTATGATACCGTCCTTCCCACAGAATCTCTTCCAGC
TGAACCTTTTTGTATTATCTTCCCCATGTGCTGAGAGGGACTTAACAGCTTCAAAAGCTG
CTCCAAGGAGAATTTTGTCATGAGATAGAGACGTTTTCCGAGTCTCTTGGCTATATATTT
CCACAGCATTCTGTCCATGAGCCAGCCAATCAACCGGAGCCACATTCACCGCTTCTGCAC
AGTTGAATCCGCAGTTGAAACCCGAATGATATGCTCTAGGGAATGTTAGAACATACTCCC
CTGCATTCTGAACAGCTCGATNAACCGGGACTCCCTCATTTTTCAAAATCGTTGGAGAAA
ACTGAGTAACCAGTTCATGAAGTAGATCAGGTTGTTCATCGAACAAATCTGGTAGATGTT
TTCTCATTGCCTTCTCTAGCCCAGTTGCATGGCTTCCCGGAACTCCNTACCATACTTTTG
GCTCGCCNAAGTGA
   
Sequence Name 27-K101750-0022-663-C11C-BG29
GenBank Accession LN498323 [GenBank]
Sequencing primer gene specific primer, BG29: 5'-TCACTGAACTATCATCACTTTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K101750-0022-663-C11C-BG29
TCGGGAGCCTGCACTGGGCTAGAGAAGGCAATGAGAAAACATCTACCAGATTTGTTCGAT
GAACAACCTGATCTACTTCATGAACTGGTTACTCAGTTTTCTCCAACGATTTTGAAAAAT
GAGGGAGTCCCGGTTTATCGAGCTGTTCAGAATGCAGGGGAGTATGTTCTAACATTCCCT
AGAGCATATCATTCGGGTTTCAACTGCGGATTCAACTGTGCAGAAGCGGTGAATGTGGCT
CCGGTTGATTGGCTGGCTCATGGACAGAATGCTGTGGAAATATATAGCCAAGAGACTCGG
AAAACGTCTCTATCTCATGACAAAATTCTCCTTGGAGCAGCTTTTGAAGCTGTTAAGTCC
CTCTCAGCACATGGGGAAGATAATACAAAAAGGTTCAGCTGGAAGAGATTCTGTGGGAAG
GACGGTATCATAACTAAGGCCATCGAGGCACGGTTACGGATGGAAGAAAAAAGAATTGAG
GCTCTTGGAAATGGTTTCAGCTTGGTAAAGATGGATAAAGACTTTGATTCAAACTGTGAA
AGGGAATGCATCTCTGGCTTTAGTGACTTGCATCTCTCTGCTACTGGCTGCAAGAATTGC
TCATCTCTCGAAGAATATGGTTGCACGAAACACGATATTTGTTCATGTGAAGGGAAAGAC
CGTTTCATTTTTCTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCANAAATGTAG

Last Updated on 06.12.2018 16:11