Sequences from confirmation amplicon


Confirmation sequences for line 664F12 and hit At4g16765 are shown.
Line ID 664F12
Locus At4g16765 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9430617
Primers used for PCR 36vf - 8474, show primer details
   
Sequence Name 16-K117874-0022-664-F12C-36vf
GenBank Accession LN493614 [GenBank]
Sequencing primer gene specific primer, 36vf: 5'-TGAATGATCCTACAGCAGTTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117874-0022-664-F12C-36vf
GTTCTGGTTGGCTTGATATTAATCTTAATGAACTTTGGTCAGAGTTAGATCGTAACATAT
GTAATCAGTGGAAACTTAAGCCACATTCCGGGAGTTTGAGTCAGGTTTTGATCTCTGCAC
TTGTTATGATTATAGGTGAAGTGATTTCGTCAGATGTTGAAACGTATGGTGCCTCAGCTC
ACTCAGATTATGGAATGGTCACTCTTCTTTTGACTGATGGAGTTCCAGGACTTCAGGTCA
ATATCTCTGAATTTCTCGTCATAATCTTCTATTAGTAACCTTATGTTCTTACACTGTCTT
TGAACTGAACTCTTAGGTTTGTAGAGACAAATCGAAACAACCACACATTTGGGAAGATGT
CCCAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCC
   
Sequence Name 04-K117873-0022-664-F12C-8409
GenBank Accession LN488098 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117873-0022-664-F12C-8409
TTGGGACATCTTCCCAAATGTGTGGTTGTTTCGATTTGTCTCTACAAACCTAAGAGTTCA
GTTCAAAGACAGTGTAAGAACATAAGGTTACTAATAGAAGATTATGACGAGAAATTCAGA
GATATTGACCTGAAGTCCTGGAACTCCATCAGTCAAAAGAAGAGTGACCATTCCATAATC
TGAGTGAGCTGAGGCACCATACGTTTCAACATCTGACGAAATCACTTCACCTATAATCAT
AACAAGTGCAGAGATCAAAACCTGACTCAAACTCCCGGAATGTGGCTTAAGTTTCCACTG
ATTACATATGTTACGATCTAACTCTGACCAAAGTTCATTAAGATTAATATCAAGCCAACC
AGAACTTTTTGATGTACATAGACACACCTGGATATCGTAAGAGGCGAACAACTGCTGTAG
AATCATTC

Last Updated on 06.12.2018 16:11