Sequences from confirmation amplicon


Confirmation sequences for line 664H09 and hit At2g43860 are shown.
Line ID 664H09
Locus At2g43860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18163314
Primers used for PCR 01vs - 8474, show primer details
   
Sequence Name 28-K118269-0022-664-H09C-01vs
GenBank Accession LN499039 [GenBank]
Sequencing primer gene specific primer, 01vs: 5'-CACTCCGTCCATATCACTAACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118269-0022-664-H09C-01vs
CGGTCCAGATCCACTAATGTATTCATACAAACCATTCGATGCGGTCCAGGCCACGGCATC
AGGTAACACACACATACATTTCTTGCTCCCCATCTTTCTTGATATTTCACTTTCTAAAAT
GTTCTCTTGTTTTTTTTGTTTGTGTGAACTCTCCAACAGTATTGGAAGTCTTGGACGAGC
AGAGGAAGAACAAGGAGTAGATAATGTGACCGTGAGTAACGTGGATTTCATGGGAACGAA
TAACGGAGTTAGGATCAAAACATGGGGGAAAGATAGCAACAGTTTCGCTAGAAACATTGT
TTTCCAACATATTAACATGAAGATGGTCAAGAACCCAATCATTATAAACCTTGCCCTAAA
CAAGGTAAGAATATTTATTAATTAACCATATCTTTAAACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 13-K118268-0022-664-H09C-8409
GenBank Accession LN492249 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118268-0022-664-H09C-8409
ATTTACATTGAATATATCCTGTTTAAAGATATGGTTAATTAATAAATATTCTTACCTTGT
TTAGGGCAAGGTTTATAATGATTGGGTTCTTGACCATCTTCATGTTAATATGTTGGAAAA
CAATGTTTCTAGCGAAACTGTTGCTATCTTTCCCCCATGTTTTGATCCTAACTCCGTTAT
TCGTTCCCATGAAATCCACGTTACTCACGGTCACATTATCTACTCCTTGTTCTTCCTCTG
CTCGTCCAAGACTTCCAATACTGTTGGAGAGTTCACACAAACAAAAAAAACAAGAGAACA
TTTTAGAAAGTGAAATATCAAGAAAGATGGGGAGCAAGAAATGTATGTGTGTGTTACCTG
ATGCCGTGGCCTGGACCGCATCGAATGGTTTGTATGAATACATTAGTGGATCCTGGACCG
ATGGAGATGCAATCATCACCGGTTCCGATTCTTGAGTTAGTGATTGGAACCGGAGTG

Last Updated on 06.12.2018 16:11